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| Ramos,Márcio Viana; Grangeiro,Thalles Barbosa; Cavada,Benildo Sousa; Shepherd,Iain; Lopes,Roberval Oliveira de Melo; Sampaio,Alexandre Holanda. |
| The lectins, proteins which specifically recognize carbohydrate moieties, have been extensively studied in many biochemical and structural aspects in order to establish the molecular basis of this non-catalytic event. On the other hand, their clinical and agricultural potentials have been growing fast. Although lectins, mainly those from legume plants, had been investigated for biological properties, studies about the physiological functions of lectins are scarce in literature. Therefore, despite the accumulated data on lectins (as proteins), the role played by these signalizing molecules is poorly discussed. In the light of our accumulated results on legume lectins, specially those obtained from plants belonging to the Diocleinae sub-tribe and available... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Binding-site; Biological functions; Fine sugar-specificity; Legume lectins. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000400001 |
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| Ramos,Márcio Viana; Bomfim,Liezelotte Rezende; Bandeira,; Debray,Henri. |
| Cratylia floribunda seeds were ground and the clean crude saline extract was fractionated into albumin, globulin, prolamin, acidic and basic glutelin protein fractions. These protein fractions were examined for the presence of an endogenous lectin receptor by SDS-polyacrylamide gel electrophoresis, western blot, affinity chromatography on a Sepharose 4B-Cratylia floribunda (CFL) lectin column and kinetic analysis in real time by surface plasmon resonance (SPR). Prolamin was the richest protein fraction although very poor in haemagglutinating activity. Basic glutelin was far the less interesting fraction for lectin activity and protein content, even though this fraction contains considerable amounts of carbohydrates. Lectin was present in all protein... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Protein-carbohydrate interaction; Surface plasmon resonance; Western blot. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000300003 |
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