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	Provedor de dados BJMBR (2) Autor Ramos,M.C. (2) Oliveira,A.M. (1) Panunto,A.C. (1) Villares,M.C.B. (1) Palavra-chave Genotyping (2) AIDS (1) IS1245 RFLP (1) Methicillin-resistant Staphylococcus aureus (1) Mycobacteria (1) Mycobacterium avium (1) PCR ribotyping (1) Staphylococcus aureus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2003 (1) 2002 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última IS1245 restriction fragment length polymorphism typing of Mycobacterium avium from patients admitted to a reference hospital in Campinas, Brazil BJMBR Panunto,A.C.; Villares,M.C.B.; Ramos,M.C.. Mycobacterium avium is an important pathogen among immunodeficient patients, especially patients with AIDS. The natural history of this disease is unclear. Several environmental sources have been implicated as the origin of this infection. Polyclonal infection with this species is observed, challenging the understanding of its pathogenesis and treatment. In the present study 45 M. avium strains were recovered from 39 patients admitted to a reference hospital between 1996 and 1998. Species identification was performed using a species-specific nucleic acid hybridization test (AccuProbe®) from Gen-Probe®. Strains were genotyped using IS1245 restriction fragment length polymorphism typing. Blood was the main source of the organism. In one patient with... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium avium; IS1245 RFLP; Mycobacteria; AIDS; Genotyping. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001000017 PCR-based ribotyping of Staphylococcus aureus BJMBR Oliveira,A.M.; Ramos,M.C.. Genotyping techniques are valuable tools for the epidemiologic study of Staphylococcus aureus infections in the hospital setting. Pulsed-field gel electrophoresis (PFGE) is the current method of choice for S. aureus strain typing. However, the method is laborious and requires expensive equipment. In the present study, we evaluated the natural polymorphism of the genomic 16S-23S rRNA region for genotyping purpose, by PCR-based ribotyping. Three primer pairs were tested to determine the size of amplicons produced and to obtain better discrimination with agar gel electrophoresis and ethidium bromide staining. The resolution of the typing system was determined using sets of bacteria obtained from clinical specimens from a large tertiary care hospital. These... Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; PCR ribotyping; Genotyping; Methicillin-resistant Staphylococcus aureus. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000200005 Registros recuperados: 2 Primeira ... 1 ... Última

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