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	Provedor de dados Ciênc. Tecnol. Aliment. (2) BJM (1) Autor Ramos,Roberta Juliano (3) Vieira,Cleide Rosana Werneck (2) Aleixo,José Antonio Guimarães (1) Baseggio,Andressa Mara (1) Carvalhal,José Beiro (1) Cattani,Cristhiane Stecanella de Oliveira (1) Cirolini,Andréia (1) Conceição,Rita de Cássia dos Santos da (1) Faria,Renata D'Aquino (1) Goularte,Fabiana Lemos (1) Miotto,Letícia Adélia (1) Miotto,Marília (1) Moreira,Ângela Nunes (1) Pereira,Murilo Anderson (1) Silveira Junior,Nelson (1) Mais... Palavra-chave Alternative methods (1) Bivalve mollusks (1) Conventional methods (1) Escherichia coli (1) Filter-feeders (1) Immunomagnetic separation (1) Microbial contamination (1) Microbiological quality (1) Milk (1) Rapid methods (1) Salmonella (1) Vibrio parahaemolyticus (1) Vibrio vulnificus (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2013 (1) 2012 (1) 2008 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Detection of salmonella sp in chicken cuts using immunomagnetic separation BJM Conceição,Rita de Cássia dos Santos da; Moreira,Ângela Nunes; Ramos,Roberta Juliano; Goularte,Fabiana Lemos; Carvalhal,José Beiro; Aleixo,José Antonio Guimarães. The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional plating in selective agar (IMS-plating). First, protein A-coated microspheres were sensitized with polyclonal antibodies against lipopolysacharide and flagella from salmonellae and used to standardize a procedure for capturing Salmonella Enteritidis from pure cultures and detection in selective agar. Subsequently, samples of chicken meat experimentally contaminated with S.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Salmonella; Immunomagnetic separation; Rapid methods. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100034 Ocurrence of Vibrio spp., positive coagulase staphylococci and enteric bacteria in oysters (Crassostrea gigas) harvested in the south bay of Santa Catarina island, Brazil Ciênc. Tecnol. Aliment. Ramos,Roberta Juliano; Pereira,Murilo Anderson; Miotto,Letícia Adélia; Faria,Renata D'Aquino; Silveira Junior,Nelson; Vieira,Cleide Rosana Werneck. The aim of this study was to assess the contamination of oysters (Crassostrea gigas), harvested in six different regions of the South Bay of Santa Catarina Island, with Coliforms at 45 ºC, Escherichia coli, Vibrio spp., positive coagulase staphylococci, and Salmonella sp. over a period of one year. One hundred eighty oyster samples were collected directly from their culture sites and analyzed. Each sample consisted of a pool of 12 oysters. All of the samples analyzed showed absence of Salmonella, 18 (10%) samples showed presence of Escherichia coli, 15 (8.3%) samples were positive for V. alginolyticus, and Vibriocholerae was detected in 4 samples (2.2%). The counts of positive-coagulase staphylococci varied from <10 to 1.9 x 102 CFU.g-1, whereas the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbiological quality; Bivalve mollusks; Filter-feeders; Escherichia coli; Vibrio parahaemolyticus; Vibrio vulnificus. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000300009 Evaluation of the PetrifilmTM and TEMPO® systems and the conventional method for counting microorganisms in pasteurized milk Ciênc. Tecnol. Aliment. Cirolini,Andréia; Baseggio,Andressa Mara; Miotto,Marília; Ramos,Roberta Juliano; Cattani,Cristhiane Stecanella de Oliveira; Vieira,Cleide Rosana Werneck. New microbiological methods have been developed and commercialized, but their performance must be guaranteed. The aim of the present study was to evaluate the PetrifilmTM and TEMPO® systems compared to the conventional method for counting microorganisms in pasteurized milk. A total of 141 samples of pasteurized milk were analyzed by counting mesophilic aerobic, Coliforms at 35 ºC, Coliforms at 45 ºC, and Escherichia coli microorganisms. High correlation was found between the methods for counting Coliforms at 35 ºC, but low correlation was found for counting mesophilic aerobic, Coliforms at 45 ºC, and Escherichia coli. No significant statistical difference was found among the three methods for counting Coliforms at 35 ºC; however, the mean counts of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternative methods; Milk; Microbial contamination; Conventional methods. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000400026 Registros recuperados: 3 Primeira ... 1 ... Última

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