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| Danesi,Eliane Dalva Godoy; Rangel-Yagui,Carlota Oliveira; Sato,Sunao; Carvalho,João Carlos Monteiro de. |
| The effects of light intensity and temperature in S. platensis cultivation with potassium nitrate or urea as nitrogen source were investigated, as well as the biomass chlorophyll contents of this cyanobacteria, through the Response Surface Methodology. Experiments were performed at temperatures from 25 to 34.5ºC and light intensities from 15 to 69 µmol photons m-2 s-1, in mineral medium. In cultivations with both sources of nitrogen, KNO3 and urea, statistic evaluation through multiple regression, no interactions of such independent variables were detected in the results of the dependent variables maximum cell concentration, chlorophyll biomass contents, cell and chlorophyll productivities, as well as in the nitrogen-cell conversion factor. In cultivation... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spirulina platensis; Light intensity; Temperature; Nitrogen sources; Chlorophyll. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100046 |
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| Santos,João Henrique Picado Madalena; Torres-Obreque,Karin Mariana; Meneguetti,Giovanna Pastore; Amaro,Beatriz Panichi; Rangel-Yagui,Carlota Oliveira. |
| The covalent attachment of polyethylene glycol (PEG) to therapeutical proteins is an important route to develop biobetters for biomedical, biotech and pharmaceutical industries. PEG conjugation can shield antigenic epitopes of the protein, reduce degradation by proteolytic enzymes, enhance long-term stability and maintain or even improve pharmacokinetic and pharmacodynamics characteristics of the protein drug. Nonetheless, correct information in terms of the PEGylation process from reaction to downstream processing is of paramount importance for the industrial application and processing scale-up. In this review we present and discuss the main steps in protein PEGylation, namely: PEGylation reaction, separation of the products and final characterization of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: PEGylation; Biobetters; Biological drugs; Polyethylene glycol; Protein purification; Site-specific PEGylation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000700408 |
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