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| Raposeiras,Ruy; Marriel,Ivanildo Evódio; Muzzi,Maria Rita Scotti; Paiva,Edilson; Pereira Filho,Israel Alexandre; Carvalhais,Lilia Costa; Passos,Raul Vinícius Magalhães; Pinto,Patrícia Pereira; Sá,Nadja Maria Horta de. |
| The objective of this work was to identify the most competitive and effective Rhizobium strains in order to increase common bean yield by nitrogen fixation as alternative or complementation to the nitrogen fertilization. Competitiveness tests were lead in axenic conditions, in Cerrado soil pots and in three field experiments, with native Rhizobium strains that were previously identified, according to their effectiveness and genetic variability. The identification of strains in nodules was performed using serological tests (axenic conditions) - agglutination and enzyme linked immunosorbent (Elisa) assays - and random amplified polymorfic DNA (RAPD) (Cerrado soil). Plant yield was determined using the dry weight (greenhouse conditions), total N and grain... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Nodule occupancy; Elisa; RAPD. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000300010 |
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| Pinto,Patrícia P.; Raposeiras,Ruy; Macedo,Andrea M.; Seldin,Lucy; Paiva,Edilson; Sá,Nadja M.H.. |
| High temperatures can affect the survival, establishment and symbiotic properties of Rhizobium strains. Bean nodulating Rhizobium strains are considered particularly sensitive because on this strains genetic recombinations and/or deletions occur frequently, thus compromising the use of these bacteria as inoculants. In this study R. tropici and R. leguminosarum bv. phaseoli strains isolated from Cerrado soils were exposed to thermal stress and the strains growth, survival and symbiotic relationships as well as alterations in their genotypic and phenotypic characteristics were analyzed. After successive thermal shocks at 45ºC for four hours, survival capacity appeared to be strain-specific, independent of thermo-tolerance and was more apparent in R. tropici... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bean nodulating Rhizobium strains; High temperatures. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141998000400012 |
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