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| Rizza,Marco Dalla; Real,Daniel; Reyno,Rafael; Porro,Valentina; Burgueño,Juan; Errico,Eugenia; Quesenberry,Kenneth H.. |
| Six species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA content; Genetic variability; Inter-SSR; Trifolium spp. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600015 |
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| Real,Daniel; Li,Guangdi D; Clark,Steve; Albertsen,Tony O; Hayes,Richard C; Denton,Matt D; DAntuono,Mario F; Dear,B.S. |
| There is an absence of drought tolerant herbaceous perennial forage legume and herb options other than lucerne (Medicago sativa L.) for environments with Mediterranean-like climates common in extensive areas of Southern Australia, the Mediterranean basin, and Chile. Therefore, a collection of 174 forage perennial legume and herb entries from 103 species and 32 genera was evaluated for adaptation in a diverse range of Mediterranean climatic environments in Southern Australia. The seasonal rainfall distribution varied from moderately to highly winter dominant with long term average annual rainfall ranging from 318 to 655 mm. The entries were rated for productivity and persistence over 3 yr. The 12 entries identified as the most promising for winter, summer,... |
| Tipo: Journal article |
Palavras-chave: Herbage yield; Legume persistence; Australian native germplasm. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000300003 |
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| Do Canto,Javier; Reyno,Rafael; Real,Daniel; Revell,Clinton. |
| Few studies have been conducted in annual and perennial forage legumes to investigate the development of hardseededness and the subsequent pattern of seed softening in temperate and subtropical regions of South America. Experiments were conducted during 2007 and 2008 in central Uruguay to follow the pattern of seed softening in 35 annual and perennial forage legumes, including three native species of Uruguay and five commercial cultivars. Newly ripened seeds of each plant material were placed in mesh packets on the soil surface in mid-summer. Samples were recovered monthly for germination tests and the proportion of residual hard seeds determined. The native species Adesmia bicolor (Poir.) DC., Adesmia securigerifolia Herter, and Ornithopus micranthus... |
| Tipo: Journal article |
Palavras-chave: Hard seeds; Adesmia; Ornithopus; Trifolium; Lotus. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392013000100006 |
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