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Inhibition of Mayaro virus replication by prostaglandin A1 and B2 in Vero cells BJMBR
Ishimaru,D.; Marcicano,F.G.P.; Rebello,M.A..
The effect of prostaglandins (PGA1 and PGB2) on the replication of Mayaro virus was studied in Vero cells. PGA1 and PGB2 antiviral activity was found to be dose-dependent. However, while 10 µg/ml PGB2 inhibited virus yield by 60%, at the same dose PGA1 suppressed virus replication by more than 90%. SDS-PAGE analysis of [35S]-methionine-labelled proteins showed that PGA1 did not alter cellular protein synthesis. In infected cells, PGA1 slightly inhibited the synthesis of protein C, while drastically inhibiting the synthesis of glycoproteins E1 and E2.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Prostaglandins; Mayaro virus; Vero cells; Protein synthesis.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900003
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Effect of prostaglandin A1 in the induction of stress proteins in Aedes albopictus cells BJMBR
Barbosa,J.A.; Rebello,M.A..
Prostaglandins are natural fatty acid derivatives with diverse physiological effects, including immune function and the control of cell growth. While the action of prostaglandins in the induction of stress proteins in vertebrate cells is well documented, their functions in invertebrate cells have been poorly investigated. The purpose of the present study was to investigate the effect of prostaglandin A1 (PGA1; 0.25, 1.25 and 12.5 µg/ml) on protein synthesis during the growth of Aedes albopictus cells. We found that PGA1 stimulates the synthesis of several polypeptides with molecular masses of 87, 80, 70, 57, 29, 27 and 23 kDa in Aedes albopictus cells. When the proteins induced by PGA1 and those induced by heat treatment were compared by polyacrylamide gel...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Prostaglandin; Stress proteins; Aedes albopictus cells.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000400004
Registros recuperados: 2
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