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Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties BJM
Guedes,Helma V.; Santos,Samuel T. dos; Perin,Liamara; Teixeira,Kátia R. dos S.; Reis,Veronica M.; Baldani,José I..
A polyphasic approach was applied to characterize 35 G. diazotrophicus isolates obtained from sugarcane varieties cultivated in Brazil. The isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ARDRA and RISARFLP techniques). Variability among the isolates was observed in relation to the carbon source use preference. Glucose and sucrose were used by all isolates in contrast to myo-inositol, galactose and ribose that were not metabolized. The results of the analysis showed the presence of two groups clustered at 68% of similarity. The genetic distance was higher when RISA-RFLP analysis was used. Analysis of 16S rDNA sequences from isolates showed that all of them belonged to the G. diazotrophicus species. Neither effect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Horizontal spread; Resistance plasmids; ESBL.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400023
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Survival of endophytic diazotrophic bacteria in soil under different moisture levels BJM
Oliveira,André L.M.; Canuto,Erineudo L.; Silva,Edmilson E.; Reis,Veronica M.; Baldani,José I..
The effects of soil moisture on the survival of three diazotrophic bacteria species (Azospirillum amazonense, Gluconacetobacter diazotrophicus and Azospirillum brasilense) were tested. Soil moisture had little influence on the survival of A. brasilense, which is considered a free-living species. On the other hand, increased soil moisture extended the survival of the endophytes A. amazonense and G. diazotrophicus. These results indicate that nitrogen-fixing endophytic species are more affected by soil moisture than associative nitrogen-fixing species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Soil survival; Gluconacetobacter diazotrophicus; Azospirillum amazonense; Azospirillum brasilense.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300005
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