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| Orsi,M.A; Doretto Jr,L; Camillo,S.C.A; Reischak,D; Ribeiro,S.A.M; Ramazzoti,A; Mendonça,A.O; Spilki,F.R; Buzinaro,M.G; Ferreira,H.L; Arns,C.W. |
| In 2003, Brazil was recognized as a pathogenic Newcastle Disease Virus (NDV) strain-free country for commercial poultry. This research was conducted in Brazil between December 2003 and March 2005 to verify the maintenance of this virulent NDV-free status. Serum samples from 5,455 flocks for commercial poultry farms were collected, comprising 81,825 broiler chickens. The farms were located in nine states of the country, grouped in three geographic regions. Serological evidence of NDV infection was detected in 28.8% of the surveyed farms. However, all fifteen viruses isolated and identified as Newcastle Disease Virus (NDV) were characterized as nonpathogenic strains, based on the Intracerebral Pathogenicity Index. These results showed that Brazil preserves... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Newcastle Disease Virus; Pathogenicity; Poultry; Biological characterization. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200017 |
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| Orsi,M.A; Doretto Jr,L; Camillo,S.C.A; Reischak,D; Ribeiro,S.A.M; Ramazzoti,A; Mendonça,A.O; Spilki,F.R; Buzinaro,M.G; Ferreira,H.L; Arns,C.W. |
| This study was carried out during 2002/2003, aiming to determine the prevalence of virulent Newcastle disease virus strains (NDV) in Brazilian commercial poultry farms. Clinical samples were obtained from the Southeastern, Southern and Central-Western regions, which comprise the main area of the Brazilian poultry production. Serum samples and tracheal and cloacal swabs of 23,745 broiler chickens from 1,583 flocks, including both vaccinated chickens and those with no vaccination information, were tested for NDV using a diagnostic ELISA kit. The seropositivity was 39.1%, and the isolation percentage by flock varied from 1.0 to 7.6%, and by region from 6.5 to 58.4%. Higher isolation rates (74.3-83.3%) were obtained after three passages in embryonated chicken... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Newcastle Disease Virus; NDV-free status; Pathogenicity; Broiler chickens; Biological characterization. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200014 |
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| Coelho,LR; Melo,RT; Monteiro,GP; Reischak,D; Mendonça,AO; Tavares,AAS; Rossi,DA. |
| ABSTRACT The analysis of Salmonella in the feces and the birds’ environment is a way of monitoring the colonization in the flocks and verifying the need for the introduction of stricter controls, in such a way that the results of the tests should be known before being sent for slaughter. The polymerase chain reaction (PCR), as well as other rapid methods represent alternatives increasingly used to detect enteric pathogens, but they need proof of effectiveness for their wide use. The aim of this study was to evaluate the equivalence between the results obtained by the methods: real-time PCR (BAX® System), Modified Rappaport-Vassiliadis Semi-solid Medium (MSRV) (ISO 6579) and the traditional method of official reference in Brazil for research of S.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BAX® System; MSRV; Salmonella Enteritidis; S. Typhimurium; Molecular biology. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200303 |
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| Orsi,MA; Doretto Júnior,L; Reischak,D; Silva,LHA da; Spilki,FR; Buzinaro,MG; Arns,CW. |
| Intracerebral pathogenicity index (ICPI) and mean death time (MDT) were determined using commercial live vaccines against Newcastle disease available in Brazil. The ICPI profiles obtained for B1 vaccine strains were nonvirulent and varied from 0 to 0.19, and their MDT was 104-116 hours. The LaSota strains had an ICPI varying between 0.02 and 0.37 and MDT from 92 to 116 hours. ICPI and MDT for the Clone 30 were 0.11 and 104 hours, respectively. For Ulster vaccines, ICPI and MDT were 0 and >150 hours; for VG-GA was 0.03 and 140 hours; and for C2, 0.04 and >144 hours. Eye drop vaccination and IM challenge, at the 1st week and the 4th week, respectively, resulted in highest protection for B1 (95-100%) and LaSota (90-100%) strains. The variability in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Newcastle disease virus (NDV) vaccines; Intracerebral pathogenicity index (ICPI); Mean death time (MDT). |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000200009 |
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