ABSTRACT The objective of the study was to determine the effect of routine intramuscular injection of oxytocin to induce milk ejection on behavior assessed in terms of stress and rectal temperature in crossbred cows (Bos indicus × Bos taurus) in the tropics of Veracruz, México. Immediately after calving, cows were milked twice a day (n = 210) and randomly assigned to receive at each milking: 10 IU (0.5 mL) of oxytocin intramuscularly (n = 70); 0.5 mL of saline solution intramuscularly (n = 70); or no injection (n = 70). The following behavioral indicators of stress were observed: trampling, kicking, tail movement, failure to eat, jumping to avoid injection, vocalization, urination, and defecation. After milking, rectal temperature (RT) was measured.... |