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	Provedor de dados BJM (1) Ciência Rural (1) Autor Resende,Maurício (2) Azevedo,Edisio Oliveira de (1) Castro,Roberto Soares (1) Flatschart,Roberto B. (1) Gouveia,Aurora Maria Guimarães (1) Leite,Rômulo Cerqueira (1) Mais... Palavra-chave Aujeszky’s disease virus (1) Caprine artrhitis-encephalitis virus (1) Diagnostics (1) Dot-blot hybridization (1) Enzyme immunoassays (1) Goat (1) Immunity (1) Polymerase chain reaction (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2002 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus BJM Flatschart,Roberto B.; Resende,Maurício. Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization. Tipo: Info:eu-repo/semantics/article Palavras-chave: Aujeszky’s disease virus; Polymerase chain reaction; Dot-blot hybridization. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013 Seroconversion and seroreactivity patterns of dairy goats naturally exposed to caprine arthritis-encephalitis virus in Brazil Ciência Rural Castro,Roberto Soares; Leite,Rômulo Cerqueira; Azevedo,Edisio Oliveira de; Resende,Maurício; Gouveia,Aurora Maria Guimarães. A labelled avidin-biotin ELISA (lab-ELISA) using repeated serum samples of goats showed a progressive seroconversion with higher seroconversion rate at the period going from the beginning of the breeding up to the last half of lactation (35.0%), compared to that recorded at the beginning of breeding (17.8%)(p<0.05). Furthermore, the seroreactivity pattern, evaluated by a lab-ELISA standard-curve with serum samples collected at 30-40 days intervals during 12 months, was caracterized by high individual variability. No seroreversion was observed and there were higher titers in the group of animals which delivered kids and established a lactation period (n=6; mean titre=913.4 units) compared to the group of goats that failed to conceived (n=4; mean... Tipo: Info:eu-repo/semantics/article Palavras-chave: Goat; Caprine artrhitis-encephalitis virus; Diagnostics; Enzyme immunoassays; Immunity. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000400009 Registros recuperados: 2 Primeira ... 1 ... Última

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