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Registros recuperados: 15
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Diverging patterns of introgression from Schistosoma bovis across S. haematobium African lineages ArchiMer
Rey, Olivier; Toulza, Eve; Chaparro, Cristian; Allienne, Jean-françois; Kincaid-smith, Julien; Mathieu-begné, Eglantine; Allan, Fiona; Rollinson, David; Webster, Bonnie L.; Boissier, Jérôme.
Hybridization is a fascinating evolutionary phenomenon that raises the question of how species maintain their integrity. Inter-species hybridization occurs between certain Schistosoma species that can cause important public health and veterinary issues. In particular hybrids between Schistosoma haematobium and S. bovis associated with humans and animals respectively are frequently identified in Africa. Recent genomic evidence indicates that some S. haematobium populations show signatures of genomic introgression from S. bovis. Here, we conducted a genomic comparative study and investigated the genomic relationships between S. haematobium, S. bovis and their hybrids using 19 isolates originating from a wide geographical range over Africa, including samples...
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Ano: 2021 URL: https://archimer.ifremer.fr/doc/00678/79020/81421.pdf
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Transcriptomic response to parasite infection in Nile tilapia (Oreochromis niloticus) depends on rearing density ArchiMer
Ellison, Amy R.; Uren Webster, Tamsyn M.; Rey, Olivier; Garcia De Leaniz, Carlos; Consuegra, Sofia; Orozco-terwengel, Pablo; Cable, Jo.
Background: Captive animal populations, be it for food production or conservation programmes, are often maintained at densities far beyond those in natural environments, which can have profound effects on behaviour, immune and stress levels, and ultimately welfare. How such alterations impact transcriptional responses to pathogen infection is a 'different kettle of fish' and remains poorly understood. Here, we assessed survival and gene expression profiles of infected fish reared at two different densities to elucidate potential functional genomic mechanisms for density-related differences in disease susceptibility. Results: Utilising a whole-transcriptome sequencing (RNAseq) approach, we demonstrate that rearing density in tilapia (Oreochromis niloticus)...
Tipo: Text Palavras-chave: Stocking density; Stress; Saprolegnia; Aquaculture; Transcriptome; Tilapia; Circadian rhythm; Disease risk.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00462/57353/78952.pdf
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Epigenetically facilitated mutational assimilation: epigenetics as a hub within the inclusive evolutionary synthesis ArchiMer
Danchin, Etienne; Pocheville, Arnaud; Rey, Olivier; Pujol, Benoit; Blanchet, Simon.
After decades of debate about the existence of non-genetic inheritance, the focus is now slowly shifting towards dissecting its underlying mechanisms. Here, we propose a new mechanism that, by integrating non-genetic and genetic inheritance, may help build the long-sought inclusive vision of evolution. After briefly reviewing the wealth of evidence documenting the existence and ubiquity of non-genetic inheritance in a table, we review the categories of mechanisms of parent-offspring resemblance that underlie inheritance. We then review several lines of argument for the existence of interactions between non-genetic and genetic components of inheritance, leading to a discussion of the contrasting timescales of action of non-genetic and genetic inheritance....
Tipo: Text Palavras-chave: Non-genetic inheritance; Inclusive heritability; Epigenetics; Central dogma; Genetic assimilation; Mutation randomness; Inclusive evolutionary synthesis.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00479/59037/62430.pdf
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De novo transcriptome assembly for Tracheliastes polycolpus, an invasive ectoparasite of freshwater fish in western Europe ArchiMer
Mathieu-begne, Eglantine; Loot, Geraldine; Blanchet, Simon; Toulza, Eve; Genthon, Clemence; Rey, Olivier.
Tracheliastes polycolpus is an ectoparasitic copepod that recently emerged in western Europe and that infects several freshwater fish species. Its recent successful spread might be due to its fascinating ability to shift to new host populations and/or species. Here, we present the first non-redundant and protein-coding de novo transcriptome assembly for T. polycolpus along with a quality assessment and reliable transcript annotations. This assembly was built from fifteen adult female parasites sampled from three different host species from a single river in southwestern France. Overall, 17,157 non-redundant contigs likely corresponding to protein-coding transcripts were identified, of which 13,093 (i.e., 76%) were successfully annotated. This assembly...
Tipo: Text Palavras-chave: Copepods; Monoxenous parasite; Parasite specificity; Curation.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00615/72737/75012.pdf
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Molecular approaches reveal weak sibship aggregation and a high dispersal propensity in a non‐native fish parasite ArchiMer
Prunier, Jérôme G.; Saint‐pé, Keoni; Blanchet, Simon; Loot, Géraldine; Rey, Olivier.
Inferring parameters related to the aggregation pattern of parasites and to their dispersal propensity are important for predicting their ecological consequences and evolutionary potential. Nonetheless, it is notoriously difficult to infer these parameters from wildlife parasites given the difficulty in tracking these organisms. Molecular‐based inferences constitute a promising approach that has yet rarely been applied in the wild. Here, we combined several population genetic analyses including sibship reconstruction to document the genetic structure, patterns of sibship aggregation, and the dispersal dynamics of a non‐native parasite of fish, the freshwater copepod ectoparasite Tracheliastes polycolpus. We collected parasites according to a hierarchical...
Tipo: Text Palavras-chave: Full‐sibs; Genetic structure; Parasite dispersal; Parentage analysis; Tracheliastes polycolpus; Transmission.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00693/80499/83694.pdf
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Epidemiological surveillance of schistosomiasis outbreak in Corsica (France): Are animal reservoir hosts implicated in local transmission? ArchiMer
Oleaga, Ana; Rey, Olivier; Polack, Bruno; Grech-angelini, Sébastien; Quilichini, Yann; Pérez-sánchez, Ricardo; Boireau, Pascal; Mulero, Stephen; Brunet, Aimé; Rogon, Anne; Vallée, Isabelle; Kincaid-smith, Julien; Allienne, Jean-françois; Boissier, Jérôme.
Environmental and anthropogenic changes are expected to promote emergence and spread of pathogens worldwide. Since 2013, human urogenital schistosomiasis is established in Corsica island (France). Schistosomiasis is a parasitic disease affecting both humans and animals. The parasite involved in the Corsican outbreak is a hybrid form between Schistosoma haematobium, a human parasite, and Schistosoma bovis, a livestock parasite. S. bovis has been detected in Corsican livestock few decades ago raising the questions whether hybridization occurred in Corsica and if animals could behave as a reservoir for the recently established parasite lineage. The latter hypothesis has huge epidemiological outcomes since the emergence of a zoonotic lineage of schistosomes...
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00504/61566/65491.pdf
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High prevalence of Schistosoma haematobium x Schistosoma bovis hybrids in schoolchildren in Cote d'Ivoire ArchiMer
Angora, Etienne K.; Allienne, Jean-francois; Rey, Olivier; Menan, Herve; Toure, Andre O.; Coulibaly, Jean T.; Raso, Giovanna; Yavo, William; N'Goran, Eliezer K.; Utzinger, Jurg; Balmer, Oliver; Boissier, Jerome.
Schistosomiasis is a neglected tropical disease, though it is highly prevalent in many parts of sub-Saharan Africa. While Schistosoma haematobium-bovis hybrids have been reported in West Africa, no data about Schistosoma hybrids in humans are available from Cote d'Ivoire. This study aimed to identify and quantify S. haematobium-bovis hybrids among schoolchildren in four localities of Cote d'Ivoire. Urine samples were collected and examined by filtration to detect Schistosoma eggs. Eggs were hatched and 503 miracidia were individually collected and stored on Whatman(R) FTA cards for molecular analysis. Individual miracidia were molecularly characterized by analysis of mitochondrial cox1 and nuclear internal transcribed spacer 2 (ITS 2) DNA regions. A...
Tipo: Text Palavras-chave: Cote d'Ivoire; Hybrids; Molecular analysis; Schistosoma bovis; Schistosoma haematobium.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00624/73604/73060.pdf
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Southern expansion of the invasive ant Wasmannia auropunctata within its native range and its relation with clonality and human activity ArchiMer
Chifflet, Lucile; Veronica Guzman, Noelia; Rey, Olivier; Andrea Confalonieri, Viviana; Alberto Calcaterra, Luis.
The little fire ant Wasmannia auropunctata, native to the Neotropics, has become a serious pest worldwide over the past 100 years. It was originally distributed from Mexico to northern Argentina and new evidence suggests a recent southern range expansion during the last 60 years reaching central Argentina. This supercolonial ant species has a polymorphic reproductive system. Some populations, mostly found in undisturbed natural environments, are characterised by a classical sexual haplodiploid reproductive system. In other populations, which mainly occur in human-modified habitats, diploid queens and haploid males are produced clonally while workers are produced sexually. Here we studied the association between the recent southern range expansion of W....
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00470/58180/78966.pdf
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Environmental DNA for detecting Bulinus truncatus : A new environmental surveillance tool for schistosomiasis emergence risk assessment ArchiMer
Mulero, Stephen; Boissier, Jérôme; Allienne, Jean‐françois; Quilichini, Yann; Foata, Joséphine; Pointier, Jean‐pierre; Rey, Olivier.
Under ongoing climate changes, the development of large‐scale monitoring tools for assessing the risk of disease emergence constitutes an urging challenge. This is particularly the case for snail‐borne diseases such as the urogenital bilharziasis that emerged in Corsica and threat European countries. The expansion of this tropical disease mainly relies on the local presence of competent snail hosts such as Bulinus truncatus. Unfortunately, very little is known about the actual repartition of freshwater snails worldwide which makes new emergences difficult to predict. In this study, we developed two ready‐to‐use environmental DNA‐based methods for assessing the distribution of B. truncatus from water samples collected in the field. We used two approaches, a...
Tipo: Text Palavras-chave: Bulinus truncatus; Corsica; DdPCR; Environmental DNA; Environmental monitoring; QPCR; Schistosomiasis.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00593/70554/68723.pdf
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Malacological survey in a bottle of water: A comparative study between manual sampling and environmental DNA metabarcoding approaches ArchiMer
Mulero, Stephen; Toulza, Eve; Loisier, Anaïs; Zimmerman, Meryl; Allienne, Jean-françois; Foata, Joséphine; Quilichini, Yann; Pointier, Jean-pierre; Rey, Olivier; Boissier, Jérôme.
To assess the effect of anthropogenic activities on ecosystems, it is of prime importance to develop new tools enabling a rapid characterization of ecological communities. Freshwater ecosystems are particularly impacted and threatened by human activities and need thorough attention to preserve their biodiversity and the ecological services they provide. Studying such ecosystems is generally difficult because the associated organisms are hard to sample and to monitor. We present a ready-to-use environmental metabarcoding protocol to characterize and monitor the freshwater gastropods communities from water samples. The efficiency of this new tool was compared to a classical malacological survey at 19 sampled sites from 10 distinct rivers distributed over...
Tipo: Text Palavras-chave: Biomonitoring; Corsica; Environmental DNA; Malacology; Metabarcoding; Snail communities.
Ano: 2021 URL: https://archimer.ifremer.fr/doc/00666/77777/79917.docx
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Prevalence and Risk Factors for Schistosomiasis among Schoolchildren in two Settings of Côte d’Ivoire ArchiMer
Angora, Etienne K.; Boissier, Jérôme; Menan, Hervé; Rey, Olivier; Tuo, Karim; Touré, Andre O.; Coulibaly, Jean T.; Méité, Aboulaye; Raso, Giovanna; N’goran, Eliézer K.; Utzinger, Jürg; Balmer, Oliver.
Schistosomiasis is a parasitic disease affecting more than 250 million people, primarily in sub-Saharan Africa. In Côte d’Ivoire both Schistosoma haematobium (causing urogenital schistosomiasis) and Schistosoma mansoni (causing intestinal schistosomiasis) co-exist. This study aimed to determine the prevalence of S. haematobium and S. mansoni and to identify risk factors among schoolchildren in the western and southern parts of Côte d’Ivoire. From January to April 2018, a cross-sectional study was carried out including 1187 schoolchildren aged 5–14 years. Urine samples were examined by a filtration method to identify and count S. haematobium eggs, while stool samples were subjected to duplicate Kato-Katz thick smears to quantify eggs of S. mansoni and...
Tipo: Text Palavras-chave: Côte d’Ivoire; Prevalence; Risk factors; Schistosoma haematobium; Schistosoma mansoni; Schistosomiasis.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00508/61953/66043.pdf
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Schistosoma haematobium-Schistosoma mansoni Hybrid Parasite in Migrant Boy, France, 2017 ArchiMer
Le Govic, Yohann; Kincaid-smith, Julien; Allienne, Jean-francois; Rey, Olivier; De Gentile, Ludovic; Boissier, Jerome.
Schistosomiasis is frequently detected in persons entering Europe. In 2017, we detected a Schistosoma mansoni-Schistosoma haematobium hybrid parasite infection in a migrant boy from Cote d'Ivoire entering France. Because such parasites might be established in Europe, as illustrated by an outbreak on Corsica Island, vectors of these parasites should be investigated.
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Ano: 2019 URL: https://archimer.ifremer.fr/doc/00615/72740/71879.pdf
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Persistent establishment of a tropical disease in Europe: the preadaptation of schistosomes to overwinter ArchiMer
Mulero, Stephen; Rey, Olivier; Arancibia, Nathalie; Mas-coma, Santiago; Boissier, Jerome.
BackgroundGlobal changes promote the spread of infectious diseases worldwide. In this context, tropical urogenital schistosomiasis is now permanently established in Corsica since its first emergence in 2013. The local persistence of the tropical pathogens (schistosomes) responsible for urogenital schistosomiasis at such latitudes might be explained by (i) the presence of its intermediate host, the snail Bulinus truncatus, (ii) the recurrent local reseeding of schistosomes by their vertebrate hosts (either human or animal) every summer, and/or (iii) the maintenance and survival of schistosomes within their snail hosts over winter.MethodsIn this study we conducted an ecological experiment to assess the ability of temperate and tropical schistosome strains to...
Tipo: Text Palavras-chave: Schistosomiasis; Bulinus truncatus; Corsica; Persistence; Schistosoma haematobium; Temperature.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00509/62088/66275.pdf
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A river runs through it: The causes, consequences, and management of intraspecific diversity in river networks ArchiMer
Blanchet, Simon; Prunier, Jérôme G.; Paz‐vinas, Ivan; Saint‐pé, Keoni; Rey, Olivier; Raffard, Allan; Mathieu‐bégné, Eglantine; Loot, Géraldine; Fourtune, Lisa; Dubut, Vincent.
Rivers are fascinating ecosystems in which the eco‐evolutionary dynamics of organisms are constrained by particular features, and biologists have developed a wealth of knowledge about freshwater biodiversity patterns. Over the last 10 years, our group used a holistic approach to contribute to this knowledge by focusing on the causes and consequences of intraspecific diversity in rivers. We conducted empirical works on temperate permanent rivers from southern France, and we broadened the scope of our findings using experiments, meta‐analyses, and simulations. We demonstrated that intraspecific (genetic) diversity follows a spatial pattern (downstream increase in diversity) that is repeatable across taxa (from plants to vertebrates) and river systems. This...
Tipo: Text Palavras-chave: Conservation genetics; Dendritic networks; Eco‐evolutionary dynamics; Ecosystem services; Intraspecific diversity.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00617/72935/71954.pdf
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Population genetic structure of Schistosoma bovis in Cameroon ArchiMer
Djuikwo-teukeng, Felicite Flore; Simo, Alain Kouam; Allienne, Jean-francois; Rey, Olivier; Ngapagna, Arouna Njayou; Tchuem-tchuente, Louis Albert; Boissier, Jerome.
BackgroundSchistosomiasis is neglected tropical parasitic disease affecting both humans and animals. Due to the human health impact, population genetic studies have focused on the three main human-infecting schistosome species: Schistosoma mansoni, S. haematobium and S. japonicum. Here we present novel data on the population genetic structure of Schistosoma bovis, a highly widespread and prevalent schistosome infecting ruminants, and therefore of veterinary importance.MethodsAdult S. bovis were sampled in the two main abattoirs of Cameroon (Yaounde and Douala). Twenty-two cows originating from four distinct localities were sampled and a total of 218 parasites were recovered. All parasites were genotyped using a panel of 14 microsatellite markers and a...
Tipo: Text Palavras-chave: Schistosoma bovis; Barcoding; Population genetic structure.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00481/59267/62448.pdf
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