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| Alvarez-Moya,Carlos; Reynoso Silva,Mónica; Villalobos Arámbula,Alma Rosa; Islas Sandoval,Alfonso; Castañeda Vasquez,Hugo; González Montes,Rosa María. |
| Glyphosate is noted for being non-toxic in fishes, birds and mammals (including humans). Nevertheless, the degree of genotoxicity is seriously controversial. In this work, various concentrations of a glyphosate isopropylamine salt were tested using two methods of genotoxicity assaying, viz., the pink mutation assay with Tradescantia (4430) and the comet assay with nuclei from staminal cells of the same plant. Staminal nuclei were studied in two different forms, namely nuclei from exposed plants, and nuclei exposed directly. Using the pink mutation assay, isopropylamine induced a total or partial loss of color in staminal cells, a fundamental criterion utilized in this test. Consequently, its use is not recommended when studying genotoxicity with agents... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glyphosate; DNA damage; Comet assay; Plant genotoxicity test; Tradescantia point mutation test. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100022 |
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| Alvarez-Moya,Carlos; Reynoso Silva,Mónica; Valdez Ramírez,Carlos; Gómez Gallardo,David; León Sánchez,Rafael; Canales Aguirre,Alejandro; Feria Velasco,Alfredo. |
| There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 µM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p < 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; DNA damage; Genotoxicity; Glyphosate. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100016 |
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