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Phytochemical and antifungal activity of anthraquinones and root and leaf extracts of Coccoloba mollis on phytopathogens BABT
Barros,Iuri Bezerra de; Daniel,Juliana Feijó de Souza; Pinto,Jurandir Pereira; Rezende,Maria Inês; Braz Filho,Raimundo; Ferreira,Dalva Trevisan.
The aim of this work was to study the phytochemical and antifungal activity of anthraquinones and root and leaf extracts of Coccoloba mollis on phytopathogens. The chemical analysis of ethanolic extracts showed a mixture of long-chain hydrocarbons, carboxyl esters and 3-taraxerone in the leaf extract. Two anthraquinones (emodin and physcion) were isolated and identified from the root extract. Phytochemical screening using the pharmacognostic methods revealed the presence of flavonoids and tannins in the leaves and roots. Anthraquinones were only found in the root extract, no alkaloids, coumarins, saponins and simple phenolics were present. The antifungal activity of C. mollis extracts and anthraquinones isolated from the root of this plant against...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antifungal; Phytochemical; Lasiodiplodia; Botryosphaeria; Coccoloba mollis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300015
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Xylanase production by Trichoderma harzianum rifai by solid state fermentation on sugarcane bagasse BJM
Rezende,Maria Inês; Barbosa,Aneli de Melo; Vasconcelos,Ana Flora Dalberto; Endo,Asae Sakurada.
Sugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp. (peroba) wood. The bagasse was washed, dried, milled and wetted with minimal salts medium and the cultures grown at 28 ± 2ºC for 7 days. Two extraction methods were tested for enzyme recovery: (A) Tween 80, 0.1% (v/v), in physiological saline, and (B) 50mM sodium acetate buffer, pH 5.0, under agitation (180rpm) for 15, 30 and 60min. After a single extraction, both extraction methods recovered an average of 15U/ml of xylanase activity, independent on the time of shaking. A second and third extraction recovered 10.4 and 6.6U/ml xylanase, respectively. The effect of volume size for extraction, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum; Xylanase; Solid state fermentation; Sugarcane bagasse.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100014
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