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Registros recuperados: 102 | |
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Robert, Rene. |
Centre autonome de production, d'élevage et de commercialisation, le bassin d'Arcachon assurait ces dernières années une part non négligeable de l'approvisionnement en naissain d'huîtres creuses des régions conchylicoles du littoral septentrional et méditerranéen de la France. |
Tipo: Text |
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Ano: 1983 |
URL: http://archimer.ifremer.fr/doc/00090/20149/17799.pdf |
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Kesarcodi-watson, Aditya; Miner, Philippe; Nicolas, Jean-louis; Asmani, Katia; Robert, Rene. |
During a series of experiments, bacteriological elements in scallop larval rearing were investigated: larvae susceptibility to pathogens as a function of their age, and the use of probiotic bacteria during larviculture. Younger larvae (d5 PF) were highly more susceptible to pathogenic-challenge than their older siblings, which were challenged at an older age (d15 PF). A challenge with 104 CFU mL−1 of V. pectenicida killed 100% of d5 PF larvae 7 days following challenge, yet killed only 9% of d15 PF larvae 9 days following challenge. Use of the probiotics Phaeobacter gallaeciensis, Alteromonas macleodii 0444 and Neptunomonas sp. 0536, provided for larger larvae, a high yield of competent larvae and, perhaps more importantly, protection against... |
Tipo: Text |
Palavras-chave: Scallop; Pecten maximus; Probiotics; Alteromonas macleodii; Neptunomonas; Phaeobacter. |
Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00210/32153/30900.pdf |
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Gonzalez, Ricardo; Mingant, Christian; Petton, Bruno; Robert, Rene. |
In contrast with the Japanese oyster Crassostrea gigas and the Manila clam Ruditapes philippinarum, Ostrea edulis seed production in the hatchery has been reported to be erratic, with sudden and unexplained larval and post-metamorphosis mortalities. Fecundity and initial larval quality have been related to broodstock conditioning, but effects on larval development and metamorphosis remain poorly understood. In addition, molluscan larval mortalities have been often associated with bacterial contamination and flow-through techniques may help to overcome this problem. Both aspects have been considered in the present work. O. edulis broodstock were conditioned at 19 °C and fed three different microalgal diets. Two were single-species diets: Rhodomonas salina... |
Tipo: Text |
Palavras-chave: Ostrea edulis; Feeding; Broodstock conditioning; Larval development; Flow-through. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00090/20093/17732.pdf |
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Poulain, C.; Lorrain, Anne; Mas, R.; Gillikin, D. P.; Dehairs, F.; Robert, Rene; Paulet, Yves-marie. |
The influences of diet and seawater dissolved inorganic carbon (DIC) on the carbon isotope composition of shell aragonite (Cinched in the Manila clam Ruditapes philippinarum reared under laboratory conditions were investigated. Clams were exposed to two successive negative carbon isotope shifts: a first shift in diet (delta C-13(phytoplankton)) and a second shift, 35 days later, in DIC (delta C-13(DIC)). Both successive shifts induced a decrease in delta C-13(shell). These results are the first to experimentally confirm an incorporation of respired carbon derived from food and carbon from DIC into shell carbonate of adult bivalves. Skeletal delta C-13 responded to changes in the delta C-13 of both diet and DIC in less than 7 days. Consequently, proxies... |
Tipo: Text |
Palavras-chave: Delta C-13(shell); DIC; Salinity; Metabolic carbon; Ruditapes philippinarum; Estuarine water; Proxy. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11443/8001.pdf |
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Robert, Rene; Vignier, Julien; Petton, Bruno. |
Under controlled conditions of food density and temperature, larval performances (ingestion, growth, survival and settlement success) of the flat oyster, Ostrea edulis, were investigated using a flow-through rearing system. In the first experiment, oyster larvae were reared at five different phytoplankton densities (70, 500, 1500, 2500 and 3500 μm3 μL−1: ≈1, 8, 25, 42 and 58 cells μL−1 equivalent TCg), and in the second, larvae were grown at four different temperatures (15, 20, 25 and 30°C). Overall, larvae survived a wide range of food density and temperature, with high survival recorded at the end of the experiments. Microalgae concentration and temperature both impacted significantly larval development and settlement success. A mixed diet of Chaetoceros... |
Tipo: Text |
Palavras-chave: Ostrea edulis; Larvae; Temperature; Feeding regime; Flow-through; DEB parameters. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00377/48817/49295.pdf |
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Cann Moisan, Christine; Nicolas, Laurence; Robert, Rene. |
In the present study, norepinephrine (NE), epinephrine (E), dopamine (DA) and 5-hydroxy-tryptamine (5HT) have been quantified by high performance liquid chromatography (HPLC) in Pecten maximus during larval and post-larval development. With average values ranging from 2 to 5 pg mug(-1) of protein, NE remained low through the whole larval life, while epinephrine (E) was undetected. DA and 5HT were the most abundant monoamines with significant variations between larval, pre-metamorphic and post-larval stages. During the first 20 days, corresponding to D larval and umboned larval stages, levels of DA and 5HT increased from 15 to 30 and 10 to 15 pg mug(-1) of protein, respectively. Then during the last week of larval life, at the approach of metamorphosis, DA... |
Tipo: Text |
Palavras-chave: Pecten maximus; Metamorphosis; Post larvae; Larvae; Monoamines. |
Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-568.pdf |
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Chretiennot-dinet, Marie-josèphe; Vaulot, Daniel; Galois, Robert; Spano, Anna-maria; Robert, Rene. |
The ingestion of 8 algal species by oyster larvae (Crassostrea gigas Thunberg, 1793) was measured by flow cytometry (FCM). In a preliminary experiment, cell size (estimated by light scatter) and chlorophyll fluorescence of 30 algal species were evaluated to select those species which could be mixed together and still be easily discriminated by FCM. Grazing experiments were carried out over 48 h with 6 and 15-day old larvae fed on 3 algal mixtures, each containing 3 different algal species. The concentration of each species was estimated at 0, 6, 12, 24 and 48 h by FCM. Grazing pressure on a given algal species was dependent upon the age of the larvae, the time of the day and the composition of the mixture. Grazing rates of older larvae were about twice... |
Tipo: Text |
Palavras-chave: Oyster larvae; Crassostrea gigas; Grazing; Algae; Selective feeding; Flow cytometry. |
Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00090/20164/17817.pdf |
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Robert, Rene; Pichot, Yves; Comps, Michel. |
From 1988 to 1989, a study has been conducted pertaining to the growth of the flat oyster Ostrea edulis, as well as Marteilia refringens and Bonamia ostreae outbreaks. Commercial mono specific farming (flat oysters), with low density or mixed (flat and hollow oysters) along with higher density, has been taking place in the Arcachon basin oceanographic sectors, where main hydrobiological parameters were acquired simultaneously. The excellent growth of the flat oyster was witnessed in mono specific farming. However this growth was average in mixed farming, probably due to higher total oyster density and interspecies competition. Regardless of the type of farming, Marteilia refringens infection was very quick and may have been related to climatic bay water... |
Tipo: Text |
Palavras-chave: Basin of Arcachon; Pathology; Bonamia ostreae; Marteilia refringens; Growth; Ostrea edulis; Bassin d'Arcachon; Pathologie; Bonamia ostreae; Marteilia refringens; Croissance; Ostrea edulis. |
Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/1990/rapport-1592.pdf |
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Pascual, Marcela; Martin, Anne-genevieve; Zampatti, Eduardo; Coatanea, Denis; Defossez, Jérôme; Robert, Rene. |
Because of diseases affecting fIat oyster Ostrea edulis, acclimatization of the Argentina oyster Ostrea puelchana has been tested in 1989-90 in five French oyster farming sites. Following ICES recommendations , hatchery produced juveniles, from parents imported from Argentina, were transferred to experimental si tes in summer 1989. Very high mortalities occurred, reaching 46 to 98% in March-Apri1 1990. They continued later on, resulting in the end of experiments in March 1991. The growth of surviving oysters was relatively poor in comparison of the local species. Parasites Marteilia refringens and Bonamia ostreae have been detected but at rates inadequate ta explain the high mortalities. AlI these results lead not to retain this species for breeding on... |
Tipo: Text |
Palavras-chave: Huître; Ostrea puelchana; Parasitologie; Marteilia refringens; Oysters; Ostrea puelchana; Parasitology; Marteilia refringens. |
Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/00018/12946/9916.pdf |
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Robert, Rene; Nicolas, Jean-louis; Conan, Rony. |
For lack of antibiotics, King scallop larviculture faces high mortalities. In the experimental mollusc hatchery of Argenton (Brittany, France), the presence of a vibrio, Vibrio pectinicida, associated with the stocks seems to be responsible for this low survival rate. As prophylactic methods are difficult to implement in the current structures (water sterilization, egg decontamination, isolation of the stocks), the addition of chloramphenicol is the only solution to protect the stocks. However, the use of this antibiotic, considered as dangerous for man, has been prohibited in animal production throughout the whole of Europe since 1994. The action of two other antibiotics, the erythromycine and the oxolinic acid, has been searched for in Pecten maximus... |
Tipo: Text |
Palavras-chave: Acide oxolinique; Erythromycine; Antibiotiques; Coquille St Jacques; Pecten maximus L.; Développement larvaire. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/publication-6199.PDF |
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His, Edouard; Borel, Michel; Robert, Rene; Laborde, Jean-louis. |
La numération des larves d'huître dans le plancton renseigne les ostréiculteurs sur la date de pose des collecteurs. La technique utilisée est très ancienne (BOURY, 1928) ; pour chaque station trois prélèvements par traits de filets pendant 10 minutes sont effectués : deux en surface (filet 200 et filet 130) et un à 1 mètre de profondeur (filet 130). Une méthode par pompage avec mesure instantanée du volume d'eau filtrée a été mise au point (prélèvements en surface et à 1 mètre de profondeur, et rétention des véligères sur filets 200). La comparaison des deux techniques met en évidence l'inadaptation de l'ancienne méthode. Le choix de la porosité (70 µm pour les filets 200) est mal adapté à la récolte des larves D (stade 1) dont le nombre peut être sous... |
Tipo: Text |
Palavras-chave: Numération; Technique; Reproduction; Crassostrea gigas; Larves; Naissain; Recrutement; Ostréiculture. |
Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/1987/acte-6444.PDF |
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Vignier, J.; Volety, A. K.; Rolton, A.; Le Goic, N.; Chu, F. -l. E.; Robert, Rene; Soudant, P.. |
The 2010 Deepwater Horizon (DWH) oil spill released millions of barrels of oil and dispersant into the Gulf of Mexico. The timing of the spill coincided with the spawning season of Crassostrea virginica. Consequently, gametes released in the water were likely exposed to oil and dispersant. This study aimed to (i) evaluate the cellular effects of acute exposure of spermatozoa and oocytes to surface slick oil, dispersed mechanically (HEWAF) and chemically (CEWAF), using flow-cytometric (FCM) analyses, and (ii) determine whether the observed cellular effects relate to impairments of fertilization and embryogenesis of gametes exposed to the same concentrations of CEWAF and HEWAF. Following a 30-min exposure, the number of spermatozoa and their viability were... |
Tipo: Text |
Palavras-chave: Deepwater Horizon oil spill; Oyster; Spermatozoa; Oocyte; Flow-cytometry. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00377/48851/51168.pdf |
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Registros recuperados: 102 | |
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