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| Sanna,Daria; Pala,Maria; Cossu,Piero; Dedola,Gian Luca; Melis,Sonia; Fresu,Giovanni; Morelli,Laura; Obinu,Domenica; Tonolo,Giancarlo; Secchi,Giannina; Triunfo,Riccardo; Lorenz,Joseph G.; Scheinfeldt,Laura; Torroni,Antonio; Robledo,Renato; Francalacci,Paolo. |
| We report a sampling strategy based on Mendelian Breeding Units (MBUs), representing an interbreeding group of individuals sharing a common gene pool. The identification of MBUs is crucial for case-control experimental design in association studies. The aim of this work was to evaluate the possible existence of bias in terms of genetic variability and haplogroup frequencies in the MBU sample, due to severe sample selection. In order to reach this goal, the MBU sampling strategy was compared to a standard selection of individuals according to their surname and place of birth. We analysed mitochondrial DNA variation (first hypervariable segment and coding region) in unrelated healthy subjects from two different areas of Sardinia: the area around the town of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breeding units strategy; MtDNA haplogroup distribution; Association studies. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200003 |
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| Nieddu,Mariella; Mezzanotte,Roberto; Pichiri,Giuseppina; Coni,Pier Paolo; Dedola,Gian Luca; Dettori,Maria Luisa; Pazzola,Michele; Vacca,Giuseppe Massimo; Robledo,Renato. |
| Abstract Two clones, Bt1 from Bos taurus and Om1 from Ovis orientalis musimon, were used as probes for hybridization on genomic DNA and on metaphase chromosomes in members of Bovini and Caprini tribes. Bt1 and Om1 are sequences respectively belonging to the 1.715 and 1.714 DNA satellite I families. Southern blots and fluorescence in situ hybridization experiments showed completely coherent results: the Bovini probe Bt1 hybridized only to members of the Bovini tribe and not to members of Caprini. Likewise, the Caprini probe Om1 hybridized only to members of the Caprini tribe and not to members of Bovini. Hybridization signals were detected in the heterochromatic regions of every acrocentric autosome, except for two pairs of autosomes from Capra hircus that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovidae; Satellite DNA; Fluorescence in situ hybridization; Pericentromeric region. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400513 |
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