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	Provedor de dados BABT (1) Autor Felix,Carlos Roberto (1) Guimarães,Valéria Monteze (1) Rezende,Sebastião Tavares de (1) Rodrigues,Marília de Castro (1) Palavra-chave Alpha-galactosidase (1) Aspergillus fumigatus (1) Raffinose oligosaccharides (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2005 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Purification and characterization of an alpha-galactosidase from Aspergillus fumigatus BABT Rezende,Sebastião Tavares de; Guimarães,Valéria Monteze; Rodrigues,Marília de Castro; Felix,Carlos Roberto. Aspergillus fumigatus secreted invertase (beta-fructofuranosidase) and alpha-galactosidase enzymatic activities able to hydrolyzing raffinose oligosaccharides (RO). alpha-Galactosidase was induced by galactose, melibiose and raffinose, but galactose was the most efficient inducer. It was purified by gel filtration and two ion exchange chromatographies and showed Mw of 54.7 kDa. The purified enzyme showed maximal activity against p-nitrophenyl-alpha-D-galactopyranoside (pNPGal) at pH 4.5-5.5 and 55 °C, and retained about 80% of the original activity after incubation for 90 minutes at 50ºC. The KM for pNPGal was 0.3 mM. Melibiose was hydrolyzed by the enzyme but raffinose was very poor substrate. Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus fumigatus; Alpha-galactosidase; Raffinose oligosaccharides. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200005 Registros recuperados: 1 Primeira ... 1 ... Última

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