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	Provedor de dados Scientia Agricola (4) Braz. J. Plant Physiol. (2) PAB (2) Genet. Mol. Biol. (1) Autor Rodriguez,Adriana Pinheiro Martinelli (9) Mendes,Beatriz Madalena Januzzi (5) Almeida,Weliton Antonio Bastos de (3) Dornelas,Marcelo Carnier (3) Mourão Filho,Francisco de Assis Alves (3) Amaral,Weber A. Neves do (1) Appezzato-da-Gloria,Beatriz (1) Costa,Maria Angélica Pereira de Carvalho (1) Filippi,Silvia Balbão (1) Krug,Maria Graziela Zagatto (1) Monteiro,Alessandra Cristina Boffino de Almeida (1) Nakazawa,Gerson Tokio (1) Pavan,Alexandra (1) Stipp,Liliane Cristina Libório (1) Mais... Palavra-chave Adventitious bud (2) Flowering (2) Genetic transformation (2) LEAFY (2) BAP (6-benzilaminopurina) (1) CONSTANS (1) Citrullus lanatus (1) Cultura de tecido (1) Cultura de tecidos (1) Data mining (1) Development (1) EST (1) Embriogênese somática (1) Eucalyptus (1) Gene expression (1) Histologia (1) Histological study (1) Improvement (1) Micrografting (1) Morfologia (1) Mais... Tipo do documento Info:eu-repo/semantics/article (8) Info:eu-repo/semantics/report (1) Ano 2005 (3) 2004 (2) 2003 (1) 2002 (1) 2001 (1) 2000 (1) Mais... País Brazil (9) Idioma Inglês (7) Português (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 9 Primeira ... 1 ... Última Identifying Eucalyptus expressed sequence tags related to Arabidopsis flowering-time pathway genes Braz. J. Plant Physiol. Dornelas,Marcelo Carnier; Rodriguez,Adriana Pinheiro Martinelli. Flowering initiation depends on the balanced expression of a complex network of genes that is regulated by both endogenous and environmental factors. The timing of the initiation of flowering is crucial for the reproductive success of plants; therefore, they have developed conserved molecular mechanisms to integrate both environmental and endogenous cues to regulate flowering time precisely. Extensive advances in plant biology are possible now that the complete genome sequences of flowering plants is available and plant genomes can be comprehensively compared. Thus, association studies are emerging as powerful tools for the functional identification of genes involved on the regulation of flowering pathways. In this paper we report the results of our search... Tipo: Info:eu-repo/semantics/article Palavras-chave: CONSTANS; Data mining; EST; Photoperiod; Vernalization. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000200009 EgLFY, the Eucalyptus grandis homolog of the Arabidopsis gene LEAFY is expressed in reproductive and vegetative tissues Braz. J. Plant Physiol. Dornelas,Marcelo Carnier; Amaral,Weber A. Neves do; Rodriguez,Adriana Pinheiro Martinelli. The EgLFY gene cloned from Eucalyptus grandis has sequence homology to the floral meristem identity gene LEAFY (LFY) from Arabidopsis and FLORICAULA (FLO) from Antirrhinum. EgLFY is preferentially expressed in the developing eucalypt floral organs in a pattern similar to that described previously for the Arabidopsis LFY. In situ hybridization experiments have shown that EgLFY is strongly expressed in the early floral meristem and then successively in the primordia of sepals, petals, stamens and carpels. It is also expressed in the leaf primordia of adult trees. The expression of the EgLFY coding region under control of the Arabidopsis LFY promoter could complement strong lfy mutations in transgenic Arabidopsis plants. These data suggest that EgLFY plays a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Eucalyptus; Flowering; LEAFY; Reproductive development. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000200006 A Floricaula/Leafy gene homolog is preferentially expressed in developing female cones of the tropical pine Pinus caribaea var. caribaea Genet. Mol. Biol. Dornelas,Marcelo Carnier; Rodriguez,Adriana Pinheiro Martinelli. In angiosperms, flower formation is controlled by meristem identity genes, one of which, FLORICAULA (FLO)/LEAFY (LFY), plays a central role. It is not known if the formation of reproductive organs of pre-angiosperm species is similarly regulated. Here, we report the cloning of a conifer (Pinus caribaea var. caribaea) FLO/LFY homolog, named PcLFY. This gene has a large C-terminal region of high similarity to angiosperm FLO/LFY orthologs and shorter regions of local similarity. In contrast to angiosperms, conifers have two divergent genes resembling LFY. Gymnosperm FLO/LFY proteins constitute a separate clade, that can be divided into two divergent groups. Phylogenetic analysis of deduced protein sequences has shown that PcLFY belongs to the LFY-like clade.... Tipo: Info:eu-repo/semantics/article Palavras-chave: LEAFY; Plant reproduction; Development; Gene expression; Flowering. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200021 In vitro organogenesis in watermelon cotyledons PAB Krug,Maria Graziela Zagatto; Stipp,Liliane Cristina Libório; Rodriguez,Adriana Pinheiro Martinelli; Mendes,Beatriz Madalena Januzzi. The objective of this work was to study the in vitro organogenesis of Citrullus lanatus, by the induction of adventitious buds in cotyledon segments cultured in medium supplemented with cytokinin. Explants were collected from one, three and five-day-old in vitro germinated seedlings, considering the distal and proximal cotyledon regions. The data obtained showed that in vitro organogenesis of watermelon occurred with higher efficiency, when cotyledon segments from the proximal region collected from three-day-old seedlings were cultivated in medium MS, supplemented with BAP (1 mg L-1) and coconut water (10%). The histological study showed that the organogenesis occurs directly, without callus formation, on epidermal and subepidermal layers of the explants.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrullus lanatus; Histological study; Adventitious bud. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000900004 Stomatal analysis of citrus somatic hybrids obtained by protoplast fusion PAB Costa,Maria Angélica Pereira de Carvalho; Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Rodriguez,Adriana Pinheiro Martinelli. The objective of this work was to evaluate leaf epidermis morphological characteristics of three citrus somatic hybrids, compared to their parents. Parental and somatic hybrid young leaves were collected and processed for scanning electron microscope observations. Citrus polyploid hybrids have fewer stomata per area and these are larger compared to their diploid parental parents. No differences in internal arrangement of the stomatal cells were detected between parental plants and somatic hybrids. Additional studies may determine if these differences will influence physiological behavior of the plants in the field. Tipo: Info:eu-repo/semantics/article Palavras-chave: Stoma; Somatic hybridization; Scanning electron microscopy; Polyploidy. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000300015 Regeneração in vitro de Passiflora suberosa a partir de discos foliares Scientia Agricola Monteiro,Alessandra Cristina Boffino de Almeida; Nakazawa,Gerson Tokio; Mendes,Beatriz Madalena Januzzi; Rodriguez,Adriana Pinheiro Martinelli. A regeneração in vitro de plantas de Passiflora suberosa foi obtida a partir de discos foliares. Folhas foram retiradas de plantas germinadas em casa de vegetação, imersas em solução comercial de hipoclorito de sódio (3:1), durante 20 minutos. Discos foliares de 0,5 cm de diâmetro, com a face adaxial em contato com o meio de cultura, foram introduzidos em placa de petri. Organogênese foi obtida utilizando-se meio MS acrescido de 0,5 ou 1,0 mg L-1 de BAP (6-benzilaminopurina). Após 4 a 8 semanas, observou-se formação de calos nas bordas dos discos foliares. Os calos formados foram transferidos para meio MSM, acrescido de 1,0 mg L-1 de GA3 (ácido giberélico), sob fotoperíodo de 16 horas. Desenvolvimento de gemas adventícias foi obtido a partir dos calos,... Tipo: Info:eu-repo/semantics/report Palavras-chave: BAP (6-benzilaminopurina); Cultura de tecido; Organogênese. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300033 Variações morfológicas de embriões somáticos obtidos a partir de inflorescências de bananeira Scientia Agricola Filippi,Silvia Balbão; Appezzato-da-Gloria,Beatriz; Rodriguez,Adriana Pinheiro Martinelli. A embriogênese somática pode ser uma alternativa para o melhoramento de espécies vegetais, quando associada à transformação genética. A regeneração de plântulas a partir de embriões somáticos é dependente de vários fatores, entre eles, a qualidade do embrião somático. Neste trabalho embriões somáticos com diferentes características morfológicas são descritos. Embriogênese somática de bananeira, cv. Grand Nain e Nanicão Jangada foi induzida a partir de inflorescências funcionalmente masculinas, em meio de indução (MI) seguido de subcultivo em meio de germinação (MG), em ausência de luz. A presença de luz na indução, não favoreceu a resposta embriogênica. Em ausência de luz, os explantes da cv. Nanicão Jangada, durante os seis meses de cultivo, sofreram... Tipo: Info:eu-repo/semantics/article Palavras-chave: Musa spp.; Embriogênese somática; Cultura de tecidos; Histologia; Morfologia. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000400010 Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments Scientia Agricola Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Pavan,Alexandra; Rodriguez,Adriana Pinheiro Martinelli. Genetic transformation allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs. The objective of this research was to establish a protocol for genetic transformation of Valencia and Natal sweet oranges (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck). Epicotyl segments of germinated in vitro plantlets (three weeks in darkness and two weeks in a 16-h photoperiod) were used as explants. These were co-cultivated with Agrobacterium tumefaciens strain EHA-105 and different experiments were done to evaluate the transformation efficiency: explants were co-cultivated with Agrobacterium for one, three or five days; explants were incubated with... Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic transformation; Organogenesis; Micrografting; Improvement. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100005 In vitro organogenesis optimization and plantlet regeneration in Citrus sinensis and C. limonia Scientia Agricola Almeida,Weliton Antonio Bastos de; Mourão Filho,Francisco de Assis Alves; Mendes,Beatriz Madalena Januzzi; Rodriguez,Adriana Pinheiro Martinelli. Exogenous genes can be introduced in plants by genetic transformation techniques. However, an efficient tissue culture system with high rates of plant recovery is necessary for gene introduction. This work aimed to define organogenesis and plant regeneration protocols for sweet orange varieties Natal, Valencia and Hamlin (Citrus sinensis L. Osbeck) and Rangpur lime (Citrus limonia L. Osbeck) which can be used in plant transformation experiments. Seeds of which teguments were removed, were germinated in vitro and maintained in the dark for three weeks, followed by one week at 16-h photoperiod (40 µmol m-2 s-1) and 27 ± 2°C. Organogenesis induction was done by introducing epicotyl segments in MT medium with 25 g L-1 sucrose and different BAP concentrations.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Adventitious bud; Rooting; Genetic transformation. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162002000100004 Registros recuperados: 9 Primeira ... 1 ... Última

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