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Genetic and phenotypic diversity of Rhizobium isolates from Southern Ecuador Ciência e Agrotecnologia
Torres-Gutiérrez,Roldán; Granda-Mora,Klever Iván; Alvarado-Capó,Yelenys; Rodriguez,Aminael Sánchez; Mogollón,Noroska Gabriela Salazar; Almeida,José Rafael de.
ABSTRACT Rhizobium-legume symbioses play relevant roles in agriculture but have not been well studied in Ecuador. The aim of this study was to characterize the genetic and phenotypic diversity of Rhizobium isolates associated with Phaseolus vulgaris from southern Ecuador. Morpho-cultural characterization, biochemical tests and physiological analyses were conducted to authenticate and determine the diversity of bacteria Rhizobium-like isolates. The genetic diversity of the isolates was determined by molecular techniques, which consisted of bacteria DNA extraction and amplification and sequencing of the 16S rRNA gene. The nodulation parameters and nitrogen fixation for P. vulgaris under greenhouse conditions were also assessed to determine the phenotypic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Diazotrophic bacteria; 16S rRNA gene; Nodulation; Indole acetic acid..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000600634
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