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| Ripoli,M.V; Rogberg-Muñoz,A; Liron,J.P; Giovambattista,G. |
| Several methods such as PCR-RFLP, OLA, DNA sequencing, PCR-SSCP and ARMS-PCR have been developed to detect the allelic variation at substitutions K232A of DGAT1, GH6.1 of GH, F279Y of GHR, R4C of LEP, I74V of FABP4, and at the transition in the TG 5´ leader sequence. Most of these methods are manual processes and therefore increase the time spent on the assay and limit the number of animals analyzed. Herein, we describe the development of pyrosequencing-based methods for the bovine DGAT1, GH, GHR, LEP, FABP4 and TG genes, whose polymorphisms have been associated with variation in carcass composition. This method was validated by analyzing DNA samples belonging to the Aberdeen Angus and Hereford breeds previously typed by PCR-SSCP, PCR-RFLP and/or DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine molecular markers; Polymorphism; Pyrosequencing; Marbling. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300005 |
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| Ripoli,M.V; Wei,S; Rogberg-Muñoz,A; Guo,B.L; Goszczynski,D.E; Fernandez,M.E; Mellucci,L; Lirón,J.P; Villarreal,E; Wei,Y.M; Giovambattista,G. |
| Genetic traceability refers to methods associated with the identification of animals and their products through DNA characterization of individuals, breeds or species. To trace breeds, it is necessary to define the breed groups to analyze, and the most appropriate molecular marker set. The selection of genetic markers depends on the gene frequency distribution, the genetic distance among breeds and the presence of private alleles. In this study, we assessed six single nucleotide polymorphisms (SNPs) located in the DGAT1, TG, LEP, GH, FABP4 and GnRHR genes, as potential genetic markers to be included into a panel for genetic traceability for the identification of breed origin associated with the bovine beef trade. The results of the genetic characterization... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breed traceability; Genetic traceability; Single nucleotide polymorphism; Chinese cattle; Argentine cattle. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300004 |
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