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Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin Electron. J. Biotechnol.
Rojas,Natalia Lorena; Cavalitto,Sebastián Fernando; Mignone,Carlos Fernando; Hours,Roque Alberto.
Protopectinases (PPases) constitute a heterogeneous group of extracellular enzymes able to release soluble pectin from insoluble protopectin in plant tissues. Geotrichum klebahnii (ATCC 42397) produces PPase-SE with endopolygalacturonase activity. PPase-SE has been used for pectin extraction and maceration of plant tissues. Here, the capacity of G. klebahnii to use different pectins as carbon and energy sources (CES) was studied, in addition to PPase-SE capacity to release pectin from lemon peel. The strain was unable to use pectin from different origins as CES. When G. klebahnii was cultivated with mixtures of different amounts of glucose and citrus pectin as CES, the biomass obtained was proportional to the initial concentration of glucose, which was...
Tipo: Journal article Palavras-chave: Carbon balance; Enzymatic pectin extraction; Microbial pectin utilization; Pectinases; Synthetic medium.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100012
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Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate Electron. J. Biotechnol.
Chesini,Mariana; Neila,Lorena Paola; de la Parra,Dante Fratebianchi; Rojas,Natalia Lorena; Contreras Esquivel,Juan Carlos; Cavalitto,Sebastian Fernando; Ghiringhelli,Pablo Daniel; Hours,Roque Alberto.
Background: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular...
Tipo: Journal article Palavras-chave: Acidophilic enzymes; Batch cultures; Inulin degradation; Submerged cultures.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300008
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Purification and Characterization of a Polygalacturonase Produced by Wickerhamomyces anomalus BABT
Martos,María Alicia; Butiuk,Ana Paula; Rojas,Natalia Lorena; Hours,Roque Alberto.
The aim of this work was to study the purification and physicochemical properties of an endo-polygalacturonase (PG) produced by Wickerhamomyces anomalus isolated from the citrus fruit peels. The enzyme was purified to homogeneity from the culture filtrate of W. anomalus grown on the yeast nitrogen base medium with glucose as carbon and energy source and citrus pectin as inductor. After anion-exchange chromatography and gel filtration chromatography, PG activity was eluted as a single peak, yielding 21% of the original activity. After dialysis and cation-exchange chromatography, only one fraction with PG activity was obtained, recovering 56% of initial enzyme activity and 1.3-fold increase in specific activity. The molecular weight of the enzyme was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wickerhamomyces anomalus; Polygalacturonase; Purification; Characterization.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400587
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