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	Provedor de dados Electron. J. Biotechnol. (2) Autor Guzmán,Fanny (2) Mercado,Luis (2) Rojas,Verónica (2) Gallardo,José A (1) Marshall,Sergio H (1) Morales-Lange,Byron (1) Valenzuela,Cristián (1) Mais... Palavra-chave Antibody (1) Caspase-3 (1) Fish apoptosis (1) Fish immunity (1) Piscirickettsia salmonis (1) Salmonid immunity (1) TNF-alpha antibody (1) Mais... Tipo do documento Journal article (2) Ano 2012 (2) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Immunological strategy for detecting the pro-inflammatory cytokine TNF-alpha in salmonids Electron. J. Biotechnol. Rojas,Verónica; Morales-Lange,Byron; Guzmán,Fanny; Gallardo,José A; Mercado,Luis. Background: Tumour necrosis factor-alpha (TNF-α) is a pro-inflammatory cytokine which exerts a variety of immunological functions in vertebrates. TNF-α has been identified and cloned in a number of teleost fish species; nevertheless, the functions displayed by this cytokine in fishes remain poorly understood, given that the low sequence identity compared to their mammalian counterpart, limit fish TNF-α detection using mammalian antibodies. Then, for fish immune response characterization is fundamental the production of specific fish anti-TNF-α antibody. Results: We have developed a monoespecific antibody against the pro-inflammatory molecule TNF-α of salmonid fish. TNF-α epitope region was identified... Tipo: Journal article Palavras-chave: Fish immunity; Salmonid immunity; TNF-alpha antibody. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500021 Development of a caspase-3 antibody as a tool for detecting apoptosis in cells from rainbow trout (Oncorhynchus mykiss) Electron. J. Biotechnol. Rojas,Verónica; Guzmán,Fanny; Valenzuela,Cristián; Marshall,Sergio H; Mercado,Luis. Background: Apoptosis is an active cell death process mediated by caspases activation, in which different extrinsic or intrinsic signalling pathways result in direct activation of effector caspases. Caspase-3 is considered to be the most important of the executioner caspases, which cause the morphological and biochemical changes detected in apoptotic cells. Different bacterial and virus pathogens have developed different strategies to survive inside the host and overcome natural protections, one of them is inducing apoptotic death in infected cells. We have demonstrated previously that Piscirickettsia salmonis activates this process in monocytes/macrophages from salmonid RTS11 cell line both by morphological and caspase detection assays; nevertheless,... Tipo: Journal article Palavras-chave: Antibody; Caspase-3; Fish apoptosis; Piscirickettsia salmonis. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000500012 Registros recuperados: 2 Primeira ... 1 ... Última

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