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| Gutiérrez-Cantú,Francisco Javier; Guerrero-Barrera,Alma Lilián; Sánchez Meraz,Wulfrano; Pozos-Guillen,Amaury de Jesús; Flores-Reyes,Héctor; Gutiérrez Robles,Eduardo Alejandro; Mariel Murga,Humberto; Romo Ramírez,Gabriel Fernando; Mariel Cárdenas,Jairo. |
| Tooth enamel is the hardest tissue in the body. The organic matrix configuration is provided by the main proteins amelogenin, ameloblastin and enamelysin (MMP20), an enzyme that helps to shape the matrix. The aim of this study was to determine by histochemistry the expression of amelogenin and enamelysin through the rough endoplasmic reticulum in the late stages of amelogenesis, and its expression in the Complexus golgiensis (Golgi complex / Golgi apparatus) in the early stages in human fetuses. In early stages a colocalization of both proteins inside the Golgi apparatus was found, being more evident the relationship between Golgi and amelogenin (99.92 %). In the late stage, a colocalization of both proteins and rugged endoplasmic reticulum was found. With... |
| Tipo: Journal article |
Palavras-chave: Rough endoplasmic reticulum; Complexus golgiensis; Golgi apparatus; Enamelysin; Amelogenin. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200008 |
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| Gutiérrez Cantú,Francisco Javier; Mariel Cárdenas,Jairo; Oliva Rodríguez,Ricardo; Romo Ramírez,Gabriel Fernando; Sánchez Méraz,Wulfrano; Mariel Murga,Humberto; Leal Tobias,Luis Armando. |
| During experiments in animal studies, it has been observed that enamelysin (MMP-20) is expressed during tooth development in the late secretory stage of amelogenesis but not in the mature enamel.The aim of this research was to determine the location of MMP-20 in human tooth germs in the different structures of the enamel organ.The detection of MMP-20 was performed by immunohistochemistry in 20 specimens obtained from human fetuses. Immunostaining of MMP-20 was observed from the presecretor stadium in stellate reticulum and intermediate stratum and in the basal portion of ameloblasts in the secretory stage in stellate reticulum, stratum intermedium, secretory ameloblasts, odontoblasts and dental papilla. The results of this research show the location of... |
| Tipo: Journal article |
Palavras-chave: Enamelysin; Immunohistochemistry; Ameloblasts; Odontoblasts. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400022 |
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