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| Roncato, Sidiane Coltro; Stangarlin, José Renato; Flores, Éder Lisandro de Moraes; Gonçalves, Edilaine Della Valentina; Dildey, Omari Dangelo Forlin; Júnior, Affonso Celso Gonçalves. |
| The identification of molecules present in plants by chromatographic techniques such as HPLC, can bring promising alternative to the control of plant diseases, since brassicas have glucosinolates, which act in plant defense. The objective of this work was to verify the presence of allyl isothiocyanate by HPLC (High Performance Liquid Chromatography Reverse Phase), using different methods of extraction. The plant material was leaves of Crambe abyssinica, dried at 45 °C for 48 h. The extraction methods used consisted of 200 mg L-1 of plant material for each method: infusion, aqueous solution, and cetonic, hydroalcoholic, methanol, hexane and chloroform solutions. The identification and quantification were by HPLC on C18 column UV at 244 nm. The experiment... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crambe abyssinica Hochst; Glicosinolato; Cromatografia. |
| Ano: 2014 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/11174 |
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| Gonçalves, Edilaine Della Valentina; Stangarlin, José Renato; Roncato, Sidiane Coltro; Dildey, Omari Dangelo Forlin; Meinerz, Cristiane Cláudia; Kuhn, Odair José. |
| - Female root-knot nematode can present compounds able to activate defense mechanisms in the vegetable. With this end, the chromatographic technique is based on the purification of molecules such as proteins, glycoproteins, nucleic acids and polysaccharides, which may present eliciting characteristics. The aim of this paper was to determine the elicitors purification methodology, from phytopathogenic nematodes, by gel filtration chromatography (GFC). Nematodes females of Meloidogyne incognita and Meloidogyne javanica were extracted, macerated, and subsequently subjected to gel filtration chromatography. For the M. incognita five protein peaks and seven glucosidic peaks, with molecular masses ranging from 8.80 to 285.95 KDa and from 65.09 to 7.47 µg glucose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meloidogyne incognita; Meloidogyne javanica; Eliciadores. |
| Ano: 2014 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/11175 |
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