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| Pronsato,Lucia; La Colla,Anabela; Ronda,Ana Carolina; Milanesi,Lorena; Boland,Ricardo; Vasconsuelo,Andrea. |
| Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Over-subculturing changes; Aged muscle cultures; Mitochondrial DNA. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000100001 |
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