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	Provedor de dados BJM (2) Electron. J. Biotechnol. (1) Autor Ronda,Srinivasa Reddy (3) Allu,Prasada Rao (1) Bokka,Chandra Sekhar (1) Ketineni,Chandrika (1) Lele,S.S. (1) Mallu,Maheswara Reddy (1) Rijal,Binod (1) Vemula,Sandeep (1) Mais... Palavra-chave Aeration (1) Biological activity (1) Cell lysis (1) Cross flow filtration (1) Fed-batch fermentation (1) Gamma linolenic acid (1) Gamma-linolenic acid (1) Light intensity (1) Oil supplementation (1) Photobioreactor (1) Purification (1) Secondary structure (1) Spirulina platensis (1) Temperature (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Journal article (1) Ano 2016 (1) 2012 (1) 2008 (1) País Brazil (2) Chile (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Culture Conditions stimulating high γ-Linolenic Acid accumulation by Spirulina platensis BJM Ronda,Srinivasa Reddy; Lele,S.S.. Gamma-linolenic acid (GLA) production by Spirulina platensis under different stress-inducing conditions was studied. Submerged culture studies showed that low temperature (25ºC), strong light intensity (6 klux) and primrose oil supplement (0.8%w/v) induced 13.2 mg/g, 14.6 mg/g and 13.5 mg linolenic acid per gram dry cell weight respectively. A careful observation of fatty acid profile of the cyanobacteria shows that, oleic acid and linoleic acid, in experiments with varying growth temperature and oil supplements respectively, helped in accumulating excess γ-linolenic acid. In addition, cultures grown at increasing light regimes maintained the γ-linolenic acid to the total fatty acid ratio(GLA/TFA) constant, despite any change in γ-linolenic acid content of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gamma linolenic acid; Light intensity; Oil supplementation; Temperature. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400018 Aeration effect on Spirulina platensis growth and γ-linolenic acid production BJM Ronda,Srinivasa Reddy; Bokka,Chandra Sekhar; Ketineni,Chandrika; Rijal,Binod; Allu,Prasada Rao. The influence of aeration on algal growth and gamma-linolenic acid (GLA) production in a bubble column photobioreactor was investigated. Studies were performed in a 20-L reactor at different aeration rates (0.2-2.5 vvm). Static, continuous, and periodic operation of air resulted in 41.9%, 88.4%, and 108% air saturation of dissolved oxygen, for which the corresponding values of GLA were 2.3, 6.5, and 7.5 mg·g-1 dry cell weight, respectively. An increase in the aeration rate from 0.2 to 2.5 vvm enhanced both the specific growth rate and GLA content under periodic sparging in the bicarbonate medium. With a 6-fold increase in the aeration rate, the GLA content of the alga increased by 69.64% (5.6-9.5 mg· g-1 dry cell weight). In addition, the total fatty acid... Tipo: Info:eu-repo/semantics/article Palavras-chave: Spirulina platensis; Aeration; Photobioreactor; Gamma-linolenic acid. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100002 Production, purification and characterization of recombinant human antithrombin III by Saccharomyces cerevisiae Electron. J. Biotechnol. Mallu,Maheswara Reddy; Vemula,Sandeep; Ronda,Srinivasa Reddy. Background: Antithrombin III (ATIII) is a protein that inhibits abnormal blood clots (or coagulation) by breaking down thrombin and factor Xa. ATIII helps to keep a healthy balance between hemorrhage and coagulation. The present work demonstrated the production, purification and characterization of recombinant human antithrombin (rhAT) from yeast Saccharomyces cerevisiae BY4741 was demonstrated. After expression of rhAT by S. cerevisiae, the biomass and rhAT concentration were analyzed through fed-batch fermentation process. Results: In fed-batch fermentation, the biomass (maximum cell dry weight of 11.2 g/L) and rhAT concentration (312 mg/L) of the expressed rhAT were achieved at 84 h of cultivation time. The maximum cell lysis efficiency (99.89%) was... Tipo: Journal article Palavras-chave: Biological activity; Cell lysis; Cross flow filtration; Fed-batch fermentation; Purification; Secondary structure. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400012 Registros recuperados: 3 Primeira ... 1 ... Última

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