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	Provedor de dados Thai Agricultural (2) Autor Payorm Cobelli (2) Poonsak Mekwatanakarn (2) Roongnapa Kawichai (2) Varapong Chamarerk (2) Palavra-chave GM rice (2) Real-time PCR (2) ข้าวดัดแปรพันธุกรรม (2) Bt63 (1) Bt63 rice (1) ข้าว Bt63 (1) ปฏิกิริยาลูกโซ่แบบในสภาพเวลาจริง (1) ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง (1) Mais... Tipo do documento Collection (2) Ano 2015 (2) País Thailand (2) Idioma Thailandês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última การตรวจวิเคราะห์สินค้าข้าวดัดแปรงพันธุกรรมด้วยปฏิกิริยาลูกโซ่ในสภาพเวลาจริง Thai Agricultural Varapong Chamarerk; Roongnapa Kawichai; Payorm Cobelli; Poonsak Mekwatanakarn. Currently, there are several reports on GM rice illegally available on market. To set up a defense measure in order to prevent GM contamination in Thai rice market, this activity was conducted by analyzing for CaMV 35S Promoter, NOS terminator and a transgene CryIA(b), which were carrying by the Bt63 rice. The Bt63 rice is an insect-resistant rice variety developed by the People Republic of China. The analyses were done by using a Real-Time PCR technique. DNA samples were extracted from rice products using a GeneScan extraction method and confirmed by a PCR using a primer pair KVM159/KVM160, which amplifies a rice species specific PLD gene encoding for an enzyme Phospholipase D. It was found that all samples could be amplified showing a PLD gene with a... Tipo: Collection Palavras-chave: GM rice; Bt63 rice; Real-time PCR; ข้าวดัดแปรพันธุกรรม; ข้าว Bt63; ปฏิกิริยาลูกโซ่แบบในสภาพเวลาจริง. Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5850 การตรวจวิเคราะห์ข้าวและสินค้าข้าวดัดแปรพันธุกรรมด้วยปฏิกิริยาลูกโซ่ ในสภาพเวลาจริง Thai Agricultural Varapong Chamarerk; Roongnapa Kawichai; Payorm Cobelli; Poonsak Mekwatanakarn. Genetically modified (GM) insecticidal rice line, Bt63 (Shanyou63), has been developed in China and found unauthorized in processed rice products in many countries. This transgenic rice contains a copy of the synthetic DNA sequence with two genes: CryIA(b) and CryIA(c). These genes encode for the respective Bt toxins which is lethal to Lepidoptera. The aim of this study was to determine the contamination of Bt63 in non-labelled commercially available processed rice products from supermarkets. In this study, a real-time Polymerase Chain Reaction (PCR) was used to simultaneously determine the contamination of Bt63. The survey was conducted on a total of 21 and 42 non-labeled samples of processed rice products collected in the years 2011 and 2012,... Tipo: Collection Palavras-chave: GM rice; Bt63; Real-time PCR; ข้าวดัดแปรพันธุกรรม; Bt63; ปฏิกิริยาลูกโซ่โพลีเมอเรสแบบเวลาจริง. Ano: 2015 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5923 Registros recuperados: 2 Primeira ... 1 ... Última

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