|
|
|
|
|
| Roquis, David; Taudt, Aaron; Geyer, Kathrin K.; Padalino, Gilda; Hoffmann, Karl F.; Holroyd, Nancy; Berriman, Matt; Aliaga, Benoit; Chaparro, Cristian; Grunau, Christoph; De Carvalho Augusto, Ronaldo. |
| Epigenetic mechanisms and chromatin structure play an important role in development. Their impact is therefore expected to be strong in parasites with complex life cycles and multiple, strikingly different, developmental stages, i.e. developmental plasticity. Some studies have already described how the chromatin structure, through histone modifications, varies from a developmental stage to another in a few unicellular parasites. While H3K4me3 profiles remain relatively constant, H3K27 trimethylation and bivalent methylation show strong variation. Inhibitors (A366 and GSK343) of H3K27 histone methyltransferase activity in S. mansoni efficiently blocked miracidium to sporocyst transition indicating that H3K27 trimethylation is required for life cycle... |
| Tipo: Text |
|
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00445/55690/71773.s001 |
| |
|
|
| Picard, Marion A. L.; Vicoso, Beatriz; Roquis, David; Bulla, Ingo; Augusto, Ronaldo C.; Arancibia, Nathalie; Grunau, Christoph; Boissier, Jerome; Cosseau, Celine. |
| Differentiated sex chromosomes are accompanied by a difference in gene dose between X/Z-specific and autosomal genes. At the transcriptomic level, these sex-linked genes can lead to expression imbalance, or gene dosage can be compensated by epigenetic mechanisms and results into expression level equalization. Schistosoma mansoni has been previously described as a ZW species (i.e., female heterogamety, in opposition to XY male heterogametic species) with a partial dosage compensation, but underlying mechanisms are still unexplored. Here, we combine transcriptomic (RNA-Seq) and epigenetic data (ChIP-Seq against H3K4me3, H3K27me3, and H4K20me1 histone marks) in free larval cercariae and intravertebrate parasitic stages. For the first time, we describe... |
| Tipo: Text |
Palavras-chave: Dosage compensation; Chromatin landscape; Histone modifications; Female heterogamety; Schistosoma mansoni. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00514/62580/66929.pdf |
| |
|
|
| Roquis, David; Lepesant, Julie M. J.; Picard, Marion A. L.; Freitag, Michael; Parrinello, Hugues; Groth, Marco; Emans, Remi; Cosseau, Celine; Grunau, Christoph. |
| Background Chromatin structure can control gene expression and can define specific transcription states. For example, bivalent methylation of histone H3K4 and H3K27 is linked to poised transcription in vertebrate embryonic stem cells (ESC). It allows them to rapidly engage specific developmental pathways. We reasoned that non-vertebrate metazoans that encounter a similar developmental constraint (i.e. to quickly start development into a new phenotype) might use a similar system. Schistosomes are parasitic platyhelminthes that are characterized by passage through two hosts: a mollusk as intermediate host and humans or rodents as definitive host. During its development, the parasite undergoes drastic changes, most notable immediately after infection of the... |
| Tipo: Text |
|
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00615/72712/71721.pdf |
| |
|
| |
|
|
| Fneich, Sara; Theron, Andre; Cosseau, Celine; Rognon, Anne; Aliaga, Benoit; Buard, Jerome; Duval, David; Arancibia, Nathalie; Boissier, Jerome; Roquis, David; Mitta, Guillaume; Grunau, Christoph. |
| Background: Adaptive evolution is not possible without the generation of phenotypic variants. The origin of these variations has been a central topic in evolutionary biology. Up to now, it was commonly accepted that standing genetic variation is the only cause of phenotypic variants. However, epigenetic information is emerging as a complementary source of heritable phenotypic variation that contributes to evolution. The relative importance of genetics and epigenetics in generating heritable phenotypic variation is nevertheless a matter of debate. Results: We used a host-parasite system to address this question. The human blood fluke Schistosoma mansoni can adapt rapidly to new intermediate snail hosts. The interaction between parasite and mollusk is... |
| Tipo: Text |
Palavras-chave: Epigenetics; Adaptive evolution; Compatibility polymorphism; Schistosoma mansoni. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00615/72708/71730.pdf |
| |
|
|
| Picard, Marion A. L.; Boissier, Jerome; Roquis, David; Grunau, Christoph; Allienne, Jean-francois; Duval, David; Toulza, Eve; Arancibia, Nathalie; Caffrey, Conor R.; Long, Thavy; Nidelet, Sabine; Rohmer, Marine; Cosseau, Celine. |
| Background Among more than 20,000 species of hermaphroditic trematodes, Schistosomatidae are unusual since they have evolved gonochorism. In schistosomes, sex is determined by a female heterogametic system, but phenotypic sexual dimorphism appears only after infection of the vertebrate definitive host. The completion of gonad maturation occurs even later, after pairing. To date, the molecular mechanisms that trigger the sexual differentiation in these species remain unknown, and in vivo studies on the developing schistosomulum stages are lacking. To study the molecular basis of sex determination and sexual differentiation in schistosomes, we investigated the whole transcriptome of the human parasite Schistosoma mansoni in a stage-and sex-comparative... |
| Tipo: Text |
|
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00358/46876/71864.pdf |
| |
|
|
|
