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	Provedor de dados BJM (2) Autor Dib,Cristina Corsi (2) Pinheiro,Sônia Regina (2) Rosário,Tatiana Reis do (2) Alves,Clebert José (1) Azevedo,Sérgio Santos de (1) Benites,Nilson Roberti (1) Higino,Severino Silvano dos Santos (1) Melville,Priscilla Anne (1) Roxo,Eliana (1) Souza,Gisele Oliveira de (1) Vasconcellos,Silvio Arruda (1) Mais... Palavra-chave Caprine tuberculosis (1) Cattle and buffalo organs (1) Mycobacterium bovis (1) Mycobacterium culture (1) PCR (1) PCR restriction enzyme analysis (1) Spoligotyping (1) Thin layer (1) Tuberculosis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis BJM Rosário,Tatiana Reis do; Dib,Cristina Corsi; Roxo,Eliana; Pinheiro,Sônia Regina; Vasconcellos,Silvio Arruda; Benites,Nilson Roberti. The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; Mycobacterium bovis; Thin layer; PCR; Cattle and buffalo organs. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100031 Mycobacterium bovis infection in goats from the Northeast region of Brazil BJM Higino,Severino Silvano dos Santos; Pinheiro,Sônia Regina; Souza,Gisele Oliveira de; Dib,Cristina Corsi; Rosário,Tatiana Reis do; Melville,Priscilla Anne; Alves,Clebert José; Azevedo,Sérgio Santos de. A total of 8,058 male and female mixed-breed goats and 1-4 years of age were slaughtered over a period of 7 months at the public slaughterhouse of Patos city, Paraíba state, in the Northeast region of Brazil; 822 animals were inspected for gross lesions of tuberculosis, and 12 (1.46%) had lesions suggestive of tuberculosis in the mammary gland, lungs, liver and mediastinal, mesenteric, submandibular, parotid and prescapular lymph nodes. Presence of granulomatous lesions was confirmed in the submandibular lymph node of one (8.3%) goat at the histopathological examination and at the mycobacterium culture the same sample was confirmed positive. Isolate was confirmed as belonging to the M. tuberculosis complex by PCR restriction enzyme analysis (PRA).... Tipo: Info:eu-repo/semantics/article Palavras-chave: Caprine tuberculosis; Mycobacterium culture; PCR restriction enzyme analysis; Spoligotyping. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400028 Registros recuperados: 2 Primeira ... 1 ... Última

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