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Chitarra,Cristiane Silva; Silva,Mayara Inácio Vincenzi da; Brandão,Laila Natasha Santos; Kagueyama,Francielle Cristina; Candido,Stefhano Luis; Rosa,Janaina Marcela Assunção; Nakazato,Luciano; Dutra,Valéria. |
ABSTRACT: Swine respiratory diseases such as atrophic rhinitis and bronchopneumonia caused by Pasteurella (P.) multocida cause important economic losses to the modern swine industry. The purpose of this study was to characterize P. multocida strains isolated from swine lungs by RAPD (Randomly Amplified Polymorphic DNA) to demonstrate their genetic diversity. Ninety-four samples of fragments from lungs with pneumonia and sixty one samples without pneumonia were collected in slaughterhouses in Mato Grosso during the period from December 2009 to March 2010. Clinical cases in 2012 and 2013 were also included in this study. Among the lung fragments with macroscopic lesions, without macroscopic lesions and clinical samples, 40.42%, 4.49% and 100% were positive... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pulmonary pasteurellosis; Swine; Pneumonia; Pasteurella multocida; RAPD. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100119 |
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Morgado,Thais Oliveira; Kagueyama,Francielle Cristina; Rosa,Janaina Marcela Assunção; Belizário,Melissa Debesa; Pacheco,Richard de Campos; Dutra,Valéria; Corrêa,Sandra Helena Ramiro; Paz,Regina Celia Rodrigues da. |
ABSTRACT: Toxoplasmosis is caused by Toxoplasma gondii, an obligatory intracellular protozoan, which establishes acute and chronic infections in birds and mammals, including humans. This note reports, for the first time, the detection and sequencing of DNA from T. gondii in the peripheral blood of a young free range giant anteater (Myrmecophaga tridactyla). For the diagnosis, the following methods were used: polymerase chain reaction (PCR) and positive serology (1:800) by means of the modified agglutination test (MAT). Since this species may be consumed by humans and predated by wild felids, its importance is emphasized as a probable source of zoonotic infection, in addition to its possible participation in the infection enzootic cycle. Although,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Myrmecophaga tridactyla; PCR; Modified agglutination test; Blood. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800501 |
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Brandão,Laila Natasha Santos; Rosa,Janaina Marcela Assunção; Kramer,Beatris; Sousa,Alessandra Tammy Hayakawa Ito de; Trevisol,Iara Maria; Silva,Virginia Santiago; Nakazato,Luciano; Dutra,Valéria. |
ABSTRACT: Wild boars (Sus scrofa) have become an important invasive species in all Brazilian regions. Increase in their population causes damage to rural properties, as they invade and destroy crops. To protect their crops and farm animals, producers hunt wild boars and often consume the product without any sanitary control, becoming exposed to various types of pathogens, including Toxoplasma gondii. Sanitary evaluations of these animals are scarce, especially in relation to the protozoan T. gondii. This study aimed to evaluate the occurrence of this pathogen in wild boars in Brazil. We analyzed 122 blood samples from wild boars (blood clots and serum), collected between 2014 and 2016 in five Brazilian states, using polymerase chain reaction (PCR) and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Toxoplasmosis; Wild boar; Zoonosis. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300500 |
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Godoy,Isabela de; Moraes,Danny Franciele da Silva Dias; Pitchenin,Letícia Camara; Rosa,Janaina Marcela Assunção; Kagueyama,Francielle Cristina; Silva,Aline de Jesus da; Dutra,Valéria; Nakazato,Luciano. |
ABSTRACT: The aim of this study was to determine the prevalence and diversity of veterinary clinical isolates of Staphylococcus and analyze their antimicrobial susceptibility. One hundred Staphylococcus spp. clinical isolates from domestic and wild animals were subjected to partial sequencing of the 16S rRNA gene to species determination. Antimicrobial susceptibility was obtained by a disk diffusion test against six antibiotics: amoxicillin (AMX), cephalexin (LEX), ciprofloxacin (CIP), erythromycin (ERY), gentamicin (GEN) and trimethoprim-sulfamethoxazole (SXT). The most common specie was S. pseudintermedius (61%, 61/100) and resistance to ERY (57%, 57/100), SXT (50%, 50/100) and AMX (46%, 46/100) was detected most frequently. In total, 40% (40/100) of... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Staphylococcus spp.; Antimicrobial resistance; Multidrug resistance.. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202148 |
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Soares,Luciana Maria Curtio; Schenkel,Diego Montagner; Rosa,Janaina Marcela Assunção; Azevedo,Ludmila Silva; Tineli,Tainara Renata; Dutra,Valéria; Colodel,Edson Moleta; Pescador,Caroline Argenta. |
ABSTRACT: Pythiosis in felines is a rare disease associated with the oomycete Pythium insidiosum. The aim of this report was to describe the macroscopic, histopathological, and molecular characteristics of P. insidiosum infection in a 2-year-old cat, with a localized invasive subcutaneous mass. The feline had an increase of volume near the anal region since it was younger. The cat died just after surgery. The necropsy was performed, and samples were collected for histopathological examination. Microscopically, the skin lesion was characterized by necro-eosinophilic dermatitis, panniculitis, and myositis surrounding negatively stained hyphal structures. In the sections stained with GMS, dark brown hyphae were clearly seen inside the affected tissue. They... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pythium insidiosum; Diagnostic; Cat. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000300450 |
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