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Overlapping of mononuclear cells derived from bone marrow in rats' intervertebral discs: an in vitro study Ciência Rural
Fontes,Erica Batista; Borges,Andréa Pacheco Batista; Pippi,Ney Luis; Rosa,Maurício; Sprada,Arícia; Togni,Monique.
In this study, bone marrow mononuclear cells (BM-MNC) derived from rats were used in order to promote intervertebral disc regeneration. These cells were isolated after centrifugation in a Ficoll-Paque™ PLUS density gradient and then placed in plastic dishes to proliferate during a period of 14 days. The BM-MNCs were previously labeled with the fluorescent membrane marker Chloromethyl-benzamidodialkylcarbocyanine (CM-DIL), and thereafter were implanted in rats' intervertebral discs explants as an in vitro experimental model. Daily analyses of the cells under a fluorescence microscope revealed morphological changes, which assumed a thin and elongated shape similar to cells that originally form the annulus fibroses. Histopathological analysis demonstrated the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesenchymal stem cells; Intervertebral disc; In vitro; Rat.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400024
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