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Azevedo,Michelle de Campos Soriani; Ramuno,Natália Mortari; Fachin,Luciana Raquel Vincenzi; Tassa,Mônica; Rosa,Patrícia Sammarco; Belone,Andrea de Faria Fernandes; Diório,Suzana Madeira; Soares,Cleverson Teixeira; Garlet,Gustavo Pompermaier; Trombone,Ana Paula Favaro. |
Abstract Leprosy, whose etiological agent is Mycobacterium leprae, is a chronic infectious disease that mainly affects the skin and peripheral nervous system. The diagnosis of leprosy is based on clinical evaluation, whereas histopathological analysis and bacilloscopy are complementary diagnostic tools. Quantitative PCR (qPCR), a current useful tool for diagnosis of infectious diseases, has been used to detect several pathogens including Mycobacterium leprae. The validation of this technique in a robust set of samples comprising the different clinical forms of leprosy is still necessary. Thus, in this study samples from 126 skin biopsies (collected from patients on all clinical forms and reactional states of leprosy) and 25 slit skin smear of leprosy... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium leprae; QPCR; Leprosy; Bacilloscopy. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100071 |
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Pedrini,Sílvia Cristina Barboza; Rosa,Patrícia Sammarco; Medri,Ísis Meri; Mourão,Guilherme; Bagagli,Eduardo; Lopes,Carlos Alberto de Magalhães. |
Leprosy is still a worldwide public health problem. Brazil and India show the highest prevalence rates of the disease. Natural infection of armadillos Dasypus novemcinctus with Mycobacterium leprae has been reported in some regions of the United States. Identification of bacilli is difficult, particularly due to its inability to grow in vitro. The use of molecular tools represents a fast and sensitive alternative method for diagnosis of mycobacteriosis. In the present study, the diagnostic methods used were bacilloscopy, histopathology, microbiology, and PCR using specific primers for M. leprae repetitive sequences. PCR were performed using genomic DNA extracted from 138 samples of liver, spleen, lymph nodes, and skin of 44 D. novemcinctus, Euphractus... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dasypus novemcinctus; Euphractus sexcinctus; Cabassous tatouay; Mycobacterium leprae; Eco-epidemiology; Wild mammals. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000100010 |
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