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| Acevedo,Francisca; Pizzul,Leticia; Castillo,María del Pilar; Rubilar,Olga; Lienqueo,María Elena; Tortella,Gonzalo; Diez,María Cristina. |
| In this study, different growth conditions of Anthracophyllum discolor Sp4 including the effect of agitation, additions of lignocellulosic support, inducer and surfactant were evaluated on the MnP production in Kirk medium using a culture system made up of the tubes containing the glass bead . The highest MnP production (1,354 U/L on day 13) was obtained when the medium was supplemented with wheat grain and 0.25 mM MnSO4 as inducer, under static conditions at 30°C. Two isoenzymes were purified (35 and 38 kDa respectively). MnP presented a maximal activity in the pH range between 4.5 and 5.5, a relatively high temperature tolerance (50ºC) and a high catalytic activity for 2,6-dimethoxyphenol and hydrogen peroxide. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: White-rot fungi; Ligninolytic enzymes; Purification; Lignocellulosic material. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600013 |
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| Diez,M. Cristina; Tortella,Gonzalo R; Briceño,Gabriela; Castillo,Maria del Pilar; Díaz,Jorge; Palma,Graciela; Altamirano,Carolina; Calderón,Carolina; Rubilar,Olga. |
| Background: The biobed is a simple biopurification system used to prevent the point-source pesticide contamination that occurs at farm level. The typical composition of the biomixture used in this system is soil, peat and straw in volumetric proportions of 1:1:2. The principal component is straw due to its positive effects on biological activity and thus pesticide degradation. However, access to straw can be limited in some regions, so it must be replaced by other more readily available lignocellulosic residues. Results: Therefore, two alternate lignocellulosic materials (barley husks and pine sawdust) were evaluated as partial substitutes for straw. The degradation of a repeatedly applied mixture of six pesticides by these alternates was assessed. The... |
| Tipo: Journal article |
Palavras-chave: Biopurification system; Biomixture; Lignocellulosic materials; Pesticide degradation. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600011 |
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| Elgueta,Sebastián; Rubilar,Olga; Lima,Nelson; Diez,M. Cristina. |
| Six strains of white-rot fungi isolated from southern Chile were evaluated for their ergosterol/biomass correlation and ligninolytic potential in solid medium to formulate pellets for Reactive Orange 165 (RO165) decolourization. The fungus Anthracophyllum discolor was selected to formulate complex pellets (fungal mycelium, sawdust, and activated carbon), coated pellets (complex pellet + alginate) and simple pellets (fungal mycelium). The activity of ligninolytic enzymes (laccase, manganese peroxidase, manganese-independent peroxidase, and lignin peroxidase) was evaluated in both the complex and coated pellets in modified Kirk medium, and the morphology of the pellets was studied using scanning electron microscopy (SEM). Complex pellets of A. discolor... |
| Tipo: Journal article |
Palavras-chave: Complex pellets; Ligninolytic enzymes; RO165 decolourization; White-rot fungi. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600008 |
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