Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados BJMBR (5) Autor Russo,M. (5) Nascimento,F.R.F. (3) Barbuto,J.A.M. (2) Ribeiro-Dias,F. (2) Curi,R. (1) Fernvik,E.C. (1) Gomes,E. (1) Hirata,M.H. (1) Hirata,R.D.C. (1) Jancar,S. (1) Nguyen,N.Y. (1) Rodríguez,D. (1) Santos,B.C. (1) Schor,N. (1) Starobinas,N. (1) Timenetsky,J. (1) Yoon,S. (1) Mais... Palavra-chave Macrophages (3) Nitric oxide (3) Cytotoxicity (2) Hydrogen peroxide (2) Autoimmune diseases (1) Cytokine (1) Graft-versus-host disease (1) Inhibitor of interferon-a (1) Interferon alpha (1) Interferon-a receptor (1) L929 tumor cells (1) MHC II (1) Membrane-anchored tumor necrosis factor (1) Method (1) Mycoplasma (1) Priming (1) Recombinant protein (1) Stress response (1) TNF (1) Transcriptional blockage (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Info:eu-repo/semantics/other (2) Ano 2003 (2) 2000 (1) 1998 (2) País Brazil (5) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Thioglycollate-elicited murine macrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells BJMBR Ribeiro-Dias,F.; Russo,M.; Nascimento,F.R.F.; Barbuto,J.A.M.; Timenetsky,J.; Jancar,S.. Macrophages are important components of natural immunity involved in inhibition of tumor growth and destruction of tumor cells. It is known that these cells can be activated for tumoricidal activity by lymphokines and bacterial products. We investigated whether YAC-1 tumor cells infected with Mycoplasma arginini stimulate nitric oxide (NO) release and macrophage cytotoxic activity. Thioglycollate-elicited macrophages from male BALB/c mice were co-cultured for 20 h with YAC-1 tumor cells infected or not with Mycoplasma arginini. The cytotoxic activity was evaluated by MTT assay and nitrite levels were determined with the Griess reagent. Thioglycollate-elicited macrophages co-cultured with noninfected YAC-1 cells showed low cytotoxic activity (34.7 ± 8.6%)... Tipo: Info:eu-repo/semantics/other Palavras-chave: Macrophages; Cytotoxicity; Mycoplasma; Tumor cells; Nitric oxide. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100010 Expression and biological activity of two recombinant polypeptides related to subunit 1 of the interferon-a receptor BJMBR Yoon,S.; Hirata,R.D.C.; Nguyen,N.Y.; Curi,R.; Russo,M.; Hirata,M.H.. Abnormal production of interferon alpha (IFN-a) has been found in certain autoimmune diseases and can be also observed after prolonged therapy with IFN-a. IFN-a can contribute to the pathogenesis of allograft rejection in bone marrow transplants. Therefore, the development of IFN-a inhibitors as a soluble receptor protein may be valuable for the therapeutic control of these diseases. We have expressed two polypeptides encoding amino acids 93-260 (P1) and 261-410 (P2) of the extracellular domain of subunit 1 of the interferon-a receptor (IFNAR 1-EC) in E. coli. The activities of the recombinant polypeptides and of their respective antibodies were evaluated using antiproliferative and antiviral assays. Expression of P1 and P2 polypeptides was achieved by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Interferon alpha; Interferon-a receptor; Inhibitor of interferon-a; Autoimmune diseases; Graft-versus-host disease; Recombinant protein. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700007 Absence of peripheral blood mononuclear cells priming in hemodialysis patients BJMBR Santos,B.C.; Starobinas,N.; Barbuto,J.A.M.; Russo,M.; Schor,N.. As a consequence of the proinflammatory environment occurring in dialytic patients, cytokine overproduction has been implicated in hemodialysis co-morbidity. However, there are discrepancies among the various studies that have analyzed TNF-alpha synthesis and the presence of peripheral blood mononuclear cell (PBMC) priming in this clinical setting. We measured bioactive cytokine by the L929 cell bioassay, and evaluated PBMC TNF-alpha production by 32 hemodialysis patients (HP) and 51 controls. No difference in TNF-alpha secretion was observed between controls and HP (859 ± 141 vs 697 ± 130 U/10(6) cells). Lipopolysaccharide (5 µg/ml) did not induce any further TNF-alpha release, showing no PBMC priming. Paraformaldehyde-fixed HP PBMC were not cytotoxic to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytokine; Stress response; Translational control; Transcriptional blockage; Priming; Membrane-anchored tumor necrosis factor. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200009 A method for multiple sequential analyses of macrophage functions using a small single cell sample BJMBR Nascimento,F.R.F.; Rodríguez,D.; Gomes,E.; Fernvik,E.C.; Russo,M.. Microbial pathogens such as bacillus Calmette-Guérin (BCG) induce the activation of macrophages. Activated macrophages can be characterized by the increased production of reactive oxygen and nitrogen metabolites, generated via NADPH oxidase and inducible nitric oxide synthase, respectively, and by the increased expression of major histocompatibility complex class II molecules (MHC II). Multiple microassays have been developed to measure these parameters. Usually each assay requires 2-5 x 10(5) cells per well. In some experimental conditions the number of cells is the limiting factor for the phenotypic characterization of macrophages. Here we describe a method whereby this limitation can be circumvented. Using a single 96-well microassay and a very small... Tipo: Info:eu-repo/semantics/article Palavras-chave: Macrophages; Nitric oxide; Hydrogen peroxide; MHC II; Method. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000900012 Cytotoxic activity of BCG-activated macrophages against L929 tumor cells is nitric oxide-dependent BJMBR Nascimento,F.R.F.; Ribeiro-Dias,F.; Russo,M.. The tumoricidal activity of activated macrophages has been attributed largely to the release of tumor necrosis factor (TNF), or to the production of reactive oxygen or nitrogen intermediates. The L929 tumor cell line (a murine fibroblast-like cell) when treated with actinomycin D (ActD) has been used to measure TNF<FONT FACE="Symbol">a</FONT> cytotoxicity. In the present study, we determined the cytotoxic activity of BCG-activated peritoneal macrophages against ActD-untreated L929 tumor cells. Furthermore, we measured the production of hydrogen peroxide (H2O2), nitric oxide (NO) and TNF by macrophages cultured in the presence or absence of L929 cells. As expected, BCG-activated macrophages produced significant amounts of H2O2 (16.0 ± 3.0 µM),... Tipo: Info:eu-repo/semantics/other Palavras-chave: Cytotoxicity; Macrophages; L929 tumor cells; Nitric oxide; TNF; Hydrogen peroxide. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001200012 Registros recuperados: 5 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco