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| RENHE,Ísis Rodrigues Toledo; PEREIRA,Danielle Braga Chelini; SÁ,Jaqueline Flaviana Oliveira de; SANTOS,Marcelo Cerqueira dos; TEODORO,Vanessa Aglaê Martins; MAGALHÃES,Fernando Antônio Resplande; PERRONE,Ítalo Tuler; SILVA,Paulo Henrique Fonseca da. |
| Abstract An analysis of nine sweetened condensed milk brands allowed for the identification of statistically significant difference in moisture, water activity (aw), soluble solids, percentage of protein, lactose crystals size, viscosity and in sensorial attributes of texture, characteristic flavor and flour texture. Relevant correlations between the composition and sensorial attributes were determined. Eighty percent of the samples presented of yeast from <0.6 logCFU.g-1 to 4.7 logCFU.g-1 and seventy percent presented of coagulase-negative staphylococcus from <3.1 log CFU.g-1 to 5.7 log CFU.g-1. These results attest the demand for more controls and investments to provide the market with a safe and standardized product. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Compositional characterization; Sensorial attributes; Microbiology; Lactose crystals. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612018000200293 |
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| MENDONÇA,Juliana França Monteiro de; VIEIRA,Felipe de Oliveira; FONSECA,Isabela; RIBEIRO,João Batista; ARCURI,Edna Froeder; BORGES,Maria de Fátima; BORGES,Cristiano Amâncio Vieira; SÁ,Jaqueline Flaviana Oliveira de; MARTINS,Marta Fonseca. |
| Abstract Real-time PCR (qPCR) has been used for rapid identification of Salmonella Typhimurium and Staphylococcus aureus in dairy foods, but is unable to differentiate viable and unviable pathogens. Ethidium bromide monoazide (EMA), a DNA-intercalating agent, can detect only viable cells because selectively enter cells considered unviable and bind to their DNA, inhibiting its amplification during qPCR. The objective was to establish a protocol for detection of viable Salmonella Typhimurium and S. aureus, experimentally inoculated in coalho cheese, by the use of EMA combined with qPCR. The protocol was effective for the identification of viable Salmonella Typhimurium in coalho cheese but not for the S. aureus cells. Concentrations of viable Salmonella... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell viability; Dairy products; DNA-intercalating agents; Foodborne diseases; Pathogenic microorganisms. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000600690 |
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