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| Sánchez-Olate,Manuel; Sáez,Patricia; Cartes,Priscila; Alvarez,Carolina; Ríos,Darcy. |
| From in vitro cultured adult material of Corylus avellana L. cv. Negretta, adventitious rooting of microshoots was evaluated. Rhizogenic induction mediated by two strains of wild-type Agrobacterium rhizogenes (A477 and A478) and indolbutyric acid (IBA) were compared under two light conditions (16:8 h photoperiod and complete darkness). The results indicate that in the 16:8 h photoperiod induction, the rooting rate with IBA (90%) was significantly higher than that obtained with the strain A477 of A. rhizogenes (67.7%), while with the strain A478 no statistically significant difference was obtained for the same variable (75%). On the other hand, under complete darkness, rooting mediated by IBA (90%) significantly surpassed the results obtained with both... |
| Tipo: Journal article |
Palavras-chave: Rooting; European hazelnut. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100013 |
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| Sánchez-Olate,Manuel; Sáez,Patricia; Ríos,Darcy. |
| The in vitro introduction of adult walnut (Juglans regia L.) tissue represents an opportunity to clone elite genotypes whose selection occurs in advanced ontogenic states. With the purpose of developing a protocol to allow mass propagation of valuable genotypes from adult material, a comparison was made between two root induction systems of walnut microshoots of the fourth subculture of adult walnut tissue of an in vitro introduction program previously reinvigorated through traditional grafting. Rhizogenic induction by indole-3-butyric acid (IBA) and Agrobacterium rhizogenes was used. The rhizogenic process was analyzed in two phases for both auxinic (T1: 3 mg L-1 IBA; T2: 5 mg L-1 IBA) and A. rhizogenes inductions (T3: A-477; T4: A-478). The first phase... |
| Tipo: Journal article |
Palavras-chave: Rooting; Microshoots; Adult material. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200019 |
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