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| TORO,ANDRÉS; ARREDONDO,CRISTIAN; CORDOVA,GONZALO; ARAYA,CLAUDIA; PALACIOS,JOSÉ L; VENEGAS,ALEJANDRO; MORITA,MASASHI; IMANAKA,TSUNEO; SANTOS,MANUEL J. |
| Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-Golgi transit in peroxisome biogenesis in mammalian cells, we evaluated the biogenesis of three peroxisomal membrane proteins (PMPs): ALDRP (adrenoleukodystrophy related protein), PMP70 and Pex3p in CHO cells. We constructed chimeric genes encoding these PMPs and green fluorescent protein (GFP), and transiently transfected them to wild type and mutant CHO cells, in which normal peroxisomes were replaced by peroxisomal membrane ghosts. The... |
| Tipo: Journal article |
Palavras-chave: Adrenoleukodystrophy; ALDRP; Endoplasmic reticulum; Peroxisome biogenesis; Pex3p and PMP70. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200014 |
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| VALENZUELA,CARLOS Y; PÉREZ-ALZOLA,PATRICIA; SANTOS,MANUEL J. |
| The maximal value of 50 % of genetic recombination (RF = recombination fraction) between two linked gene loci has been explained by two models: (i) the restriction of crossovers to only two of the four chromatids of a tetrad (tetrad model); (ii) the limit of the occurrence of an odd number of crossovers between two loci, when the expected number of crossovers tends to infinite (odd-even model). This article shows that (i) is wrong. If tetrads were responsible for the maximal 50 % RF, sister chromatid exchanges (SCE) should display a maximal 100 % RF. In mammalian CHO cells we found that SCE yield a maximal 50 % recombination. Also, we demonstrated that the maximal 50 % RF for tetrads occurs because the sum of odd order coefficients in the binomial... |
| Tipo: Journal article |
Palavras-chave: 50 % recombination; Tetrads; Odd-even crossovers; SCE. |
| Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000015 |
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