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	Provedor de dados Anais da ABC (AABC) (1) Autor MÜLLER-RÖBER,BERND (1) SURPILI,MARCELO J. (1) WILLMITZER,LOTHAR (1) Palavra-chave Library screening (1) Potassium channel (1) Signal sequences (1) Yeast acid phosphatase (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2002 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última A yeast-based model system for cloning secreted and membrane proteins Anais da ABC (AABC) SURPILI,MARCELO J.; MÜLLER-RÖBER,BERND; WILLMITZER,LOTHAR. The targeting of proteins to cell organelles and membranes, or of proteins destined to secretion, is coordinated by signal sequences located at the 5´-end of their respective genes. A signal sequence trap system was envisaged in which a truncated version of the yeast acid phosphatase pho5 gene lacking the start codon and signal sequence could serve as a reporter gene. A fraction enriched in 5´-end fragments obtained by PCR from a potato guard-cell cDNA library was cloned in frame to the acid phosphatase gene and the acid phosphatase activity was assayed directly in yeast colonies grown on selective medium. Putative signal sequences targeting the acid phosphatase to the membrane or to the outside of the cell were used to screen the cDNA bank in order to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Signal sequences; Potassium channel; Yeast acid phosphatase; Library screening. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400005 Registros recuperados: 1 Primeira ... 1 ... Última

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