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Registros recuperados: 3
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Uso de Marcadores SSR para Identificación de Germoplasma de Papa en el Programa de Mejoramiento de INIA de Chile Agricultura Técnica
Mathias R,Mónica; Sagredo D,Boris; Kalazich B,Julio.
Los marcadores moleculares basados en Secuencias Simples Repetidas (SSR) constituyen una herramienta altamente eficaz para la identificación de genotipos de papa (Solanum tuberosum L.) y pueden ser de gran utilidad en la conservación y manejo de germoplasma. Con el propósito de incorporar esta tecnología al Programa de Mejoramiento de Papa del Instituto de Investigaciones Agropecuarias (INIA) de Chile, se evaluó un grupo de 26 marcadores SSR sobre una muestra de 71 genotipos de papa. Cada marcador se caracterizó según su número de alelos y sus respectivas combinaciones, calidad de lectura, contenido de información polimórfica (PIC) y poder discriminatorio (D). Del total sólo 21 marcadores SSR mostraron productos legibles con un número de alelos que varió...
Tipo: Journal article Palavras-chave: Microsatélites; Huella digital de ADN.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072007000100001
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Implementation of a Molecular System for Traceability of Beef Based on Microsatellite Markers Chilean J. Agric. Res.
Felmer D,Ricardo; Sagredo D,Boris; Chávez R,Renato; Iraira H,Sergio; Folch M,Carolina; Parra G,Lorena; Catrileo S,Adrián; Ortiz L,Manuel.
Animal products traceability has acquired considerable importance as a security measure in EEC member countries since the food crisis of the mid-nineties. This has led to reinforcing the capacity to manage cattle product quality, with traceability emerging as the main tool to prevent risks to product security and quality as demanded by consumers in developed countries. The practical application of a traceability system for beef, based on molecular markers requires the election of a panel of microsatellites, as well as the optimization of methods of sampling and DNA analysis. In this work, a traceability system for beef based on a panel of 10 microsatellites markers was implemented. Different biological samples were evaluated, such as hair, blood, tissue...
Tipo: Journal article Palavras-chave: Traceability; Microsatellites; Individual identification; Beef.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000400004
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Evaluation of a SCAR RYSC3 Marker of the Ry adg Gene to Select Resistant Genotypes to Potato Virus Y (PVY) in the INIA Potato Breeding Program Chilean J. Agric. Res.
Sagredo D,Boris; Mathias R,Mónica; Barrientos P,Claudia; Acuña B,Ivette; Kalazich B,Julio; Santos Rojas,José.
The Potato virus Y (PVY) is distributed worldwide and is one of the most damaging viruses in terms of yield reduction in the potato (Solanum tuberosum L.) crop with losses sometimes reaching 80%. To reduce its impact, there is great interest in obtaining varieties carrying the Ry adg gene that provides extreme resistance to this virus. This could be facilitated with SCAR (sequence characterized amplified region) RYSC3 by molecular marker-assisted selection (MAS). This study compared the effectiveness of the RYSC3 marker in the detection of the Ry adg gene vs. biological tests on populations of the Potato Breeding Program of the Instituto de Investigaciones Agropecuarias (INIA), Chile. Within the group of 71 progenitors, 30 plants had some kind of...
Tipo: Journal article Palavras-chave: MAS; Extreme resistance.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000300002
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