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| Araya,Carolina; Peña,Elizabeth; Salazar,Erika; Román,Lisset; Medina,Claudia; Mora,Roxana; Aljaro,Agustín; Rosales,Inés-Marlene. |
| Big-vein disease (BVD) is a widespread and economically damaging disease in lettuce (Lactuca sativa L.). Typical symptoms are chlorotic clearing around leaf veins, leaf deformations, and impaired head development. In this research, we studied the relationship between symptom intensity and protein and viral RNA accumulation in infected plants. Naturally infected lettuce plants, from the field or greenhouse, were classified according to their symptomatology: mild, moderate, severe, and symptomless. Coat protein accumulation was evaluated by a double antibody sandwich/enzyme-linked immunosorbent assay (DAS-ELISA), and RNA levels were studied by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR. Virus coat... |
| Tipo: Journal article |
Palavras-chave: Lettuce big-vein disease; Symptom expression. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000100008 |
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| Salazar,Erika; Montenegro,Gloria. |
| The economical, environmental and social impacts associated with genetically modified (GM) crops are supported by the increased use of GM species by farmers. This increase in the use of GM species has included a global increase in both the number of hectares with GM crops and the number of countries using this technology. However, the use of GM crops has some drawbacks, due to the environmental risks associated with some of the events and social risks related to intellectual property rights issues and the monopoly associated with this technology. An overview of the current status of the use of GM crops in relation to the production, benefits, associated risks, intellectual property rights, legislation and regulatory framework is presented, focusing in the... |
| Tipo: Journal article |
Palavras-chave: Genetically modified organisms; Regulatory framework; Intellectual property; Chile. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202009000300003 |
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| Barra,Macarena; Salazar,Erika; Beltrán,María; Sagredo,Boris. |
| The extraction of high-quality DNA is essential for studies conducted at the molecular level in species with an abundance of contaminants in their tissues, such as some landraces of potato and maize, in which it is difficult to extract good-quality genomic DNA. Compounds such as polyphenols interfere with the amplification of DNA during polymerase chain reaction (PCR), which makes it difficult to conduct PCR-based studies ofmolecular markers. This article describes a simple and robust protocol for DNA isolation that was applied to potato and maize landraces and resulted in a high DNA concentration with excellent purity and quality. This method is based on the method of Krizman et al. (2006) with some modifications and was tested on potato leaves and young... |
| Tipo: Journal article |
Palavras-chave: Activated charcoal; DNA extraction; Genomic DNA; Polyphenols. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300019 |
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