|
|
|
|
|
| Ogusku,Mauricio Morishi; Sadahiro,Aya; Hirata,Mário Hiroyuki; Hirata,Rosário D. C.; Zaitz,Clarisse; Salem,Júlia Ignez. |
| Seeking to improve the laboratory diagnosis of Cutaneous Tuberculosis, a study was carried out on the application of PCR technique in macerated, decontaminated(with 4% H2SO4 for elimination of normal microbiot), neutralized (with 4% NaOH)biopsies tissues samples stored at -20ºC. Of the 37 samples submitted for study, 16.22% were positive by microscopy for acid-fast bacilli (concentrated method) and in 43.24% the Mycobacterium tuberculosis was isolated in Löwenstein-Jensen medium. Using a M. tuberculosis complex specific primer set (gene sequence for 16S rDNA), the mycobacterial DNA was detected in 24.32% of the biopsies. The sensitivity and specificity of PCR were 43.7% and 90.4%, respectively. Due to low sensitivity and discrepant results between... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium tuberculosis; Cutaneous tuberculosis; Polymerase Chain Reaction (PCR); Laboratory diagnosis. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200015 |
| |
|
|
| Lima,Alita Moura de; Salem,Júlia Ignez; Souza,João Vicente Braga de; Cortez,Ana Claudia Alves; Carvalho,Clarice Maia; Chaves,Francisco Célio Maia; Veiga Junior,Valdir Florêncio da. |
| Substances that inhibit the growth of Mycobacterium tuberculosis could potentially be used as antibiotics. These substances could also be added to test culture media to improve the speed of tuberculosis diagnosis. The aim of this work was to investigate the influence of culture filtrates of endophytic fungi isolated from P. aduncum L. on the growth of M. tuberculosis. To achieve this objective, the following methodology was used: a) endophytic fungi were isolated from the leaves and stems of P. aduncum L.; b) the isolated fungi were submitted to submerged bioprocessing; c) culture filtrates from the bioprocess were assayed to evaluate their effect on the growth of M. tuberculosis. We isolated 315 fungal types, which represented 85 morphologies, from... |
| Tipo: Journal article |
Palavras-chave: Antagonism; Stimulation growth; Tuberculosis. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400008 |
| |
|
|
|
