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Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of Mexico 118
Gutiérrez-Ibáñez,Ana T; Laguna-Cerda,Antonio; Rojas-Martínez,Reina; González-Garza,Ramiro; Salgado-Siclán,Martha L.
The Mexican potato (Solanum tuberosum L.) crop is affected by several diseases, including purple top (PPT). PPT is caused by phytoplasma and is transmitted by insects in the Cicadellidae family. In severe infections, this pathogen can cause yield losses of nearly 100%. The objective of this research was to identify and classify (at a molecular level) the causal agents of PPT. Potato plants were sampled in the state of Mexico (at Tenango del Valle, Zinacantepec, Atlacomulco and Donato Guerra) in the spring and summer of 2006. Approximately 40% of the plants tested positive for phytoplasma. The presence of plant DNA was determined by amplifying an endogenous gene with the 16s-1/16s-2 primers. To detect phytoplasma, the P1/P7 universal primers were used for...
Tipo: Journal article Palavras-chave: Phytoplasma; Potato diseases; Potato purple top.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200010
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