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| Briand, Elisabeth; Salmain, M; Compere, Chantal; Pradier, C. |
| Two antibody immobilisation procedures were compared to set up an immunosensor for goat anti-rabbit immunoglobulin (anti-rIgG), i.e. rIgG covalently bound or immobilised via affinity to protein A (PrA). In both cases, the first layer of protein was covalently bound to a mixed self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) and mercaptohexanol (C6OH) on a gold surface. The elaboration of the sensitive surfaces, as well as their selectivity and sensitivity were studied step by step by polarization modulation-reflection absorption infra-red spectroscopy (PM-RAIRS) and quartz crystal trticrobalance (QCM) with impedance measurement. QCM measurements showed that the viscoelastic properties of the antibody layer were markedly modified during the... |
| Tipo: Text |
Palavras-chave: RIgG immobilisation; PM RAIRS; QCM; Immunosensors. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3580.pdf |
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