|
|
|
|
|
| Antoine, Elisabeth; Compere, Chantal; Cosnier, Serge; Crassous, Marie-pierre; Derbal, Hassina; Erard-le Denn, Evelyne; Gondran, Chantal; Guilminot, Elodie; Lehaitre, Michel; Mazeas, Florence; Moreau, Hervé; Perrot, Hubert; Pradier, Claire-marie; Salmain, Michèle. |
| The microalgae Alexandrium minutum produces neurotoxins responsible for paralytic shellfish poisoning, which upon accumulation in shellfish represent a human health risk. This species recurrently forms toxic blooms in the Northern part of the French Brittany coast. At present, algae identification relies on tedious microscope observations mostly performed by skilled taxonomists in laboratories. Newly emerging fields of molecular taxonomy, nanoscale technology and biotechnology offer opportunities for the development of in-situ biosensors for the detection and the monitoring of toxic algae. |
| Tipo: Text |
|
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00089/19986/17650.pdf |
| |
|
|
| Briand, Elisabeth; Salmain, Michèle; Henry, Jean-marie; Perrot, Hubert; Compere, Chantal; Pradier, Claire-marie. |
| Immunosensors, based on the immobilization of a model rabbit antibody on mixed self-assembled monolayers and Protein A as a linking agent on gold transducers, were elaborated and characterized at each step by modulated polarization-infrared spectroscopy (PM-IRRAS) and occasionally by atomic force microscopy (AFM) and quartz crystal microbalance (QCM). By testing two different mixed SAMs comprising 11-mercaptoundecanoic acid (MUA), together with either decanethiol (C9CH3) or mercaptohexanol (C6OH), the role of the chemical composition and structure of the antibody attachment layer upon the sensor performance was demonstrated. (c) 2006 Elsevier B.V. All rights reserved. |
| Tipo: Text |
Palavras-chave: QCM; IRRAS; Thiol attachment; Immunosensor; Mixed SAM' s. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1988.pdf |
| |
|
|
| Briand, Elisabeth; Salmain, Michèle; Compere, Chantal; Pradier, Claire-marie. |
| Binary mixtures of 11-mercaptoundecanoic acid (MUA) and other thiols of various lengths and terminal functions were chemisorbed on gold-coated surfaces via S-Au bonds to form mixed self-assembled monolayers (SAMs). Several values of the inole fraction of MUA in the thiol mixtures were tested and the structure and composition of the resulted thin films were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). The results made it clear that co-adsorption of MUA with thiols of similar chain length led to well-ordered monolayers whereas the co-adsorption of MUA with shorter thiols yielded less crystalline-like thin films, but with more reactive carboxylic acid terminal... |
| Tipo: Text |
Palavras-chave: PM IRRAS; Antibody; Protein A; Gold; Self assembled monolayers. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2222.pdf |
| |
|
|
|
