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	Provedor de dados J. Venom. Anim. Toxins incl. Trop. Dis. (2) Autor Sampaio,RNR (2) Andreotti,R (1) Brustoloni,YM (1) Chaer,WK (1) Corvalan,FH (1) Kückelhaus,SA (1) Lima Júnior,MSC (1) Roselino,AM (1) Santos,GM (1) Mais... Palavra-chave American cutaneous leishmaniasis (1) Autochthonous cases (1) Cutaneous leishmaniasis saliva (1) Federal District (1) Leishmania Viannia braziliensis (1) Leishmania polymerase chain reaction non-invasive diagnosis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2012 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última DNA identification of Leishmania (Viannia) braziliensis in human saliva from a patient with American cutaneous leishmaniasis J. Venom. Anim. Toxins incl. Trop. Dis. Corvalan,FH; Sampaio,RNR; Brustoloni,YM; Andreotti,R; Lima Júnior,MSC. This study aims to report the amplification of the DNA of Leishmania (V.) braziliensis, using polymerase chain reaction, obtained from the saliva of a patient with American cutaneous leishmaniasis who did not present any lesion in the oral mucosa. Amplification produced fragments of 103 bp, an estimated size employing Leishmania (V.) braziliensis primers (b1 e b2). The present results revealed, for the first time, that the in vitro amplification of Leishmania DNA using samples from the salivary fluid of a patient with American cutaneous leishmaniasis is possible. However, more studies are required with a larger number of participants to evaluate the usefulness of saliva as a non-invasive sample for PCR. The development of such non-invasive technique is... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cutaneous leishmaniasis saliva; Leishmania polymerase chain reaction non-invasive diagnosis. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100013 Leishmania (Viannia) braziliensis is the main species causing cutaneous leishmaniasis in the Federal District of Brazil J. Venom. Anim. Toxins incl. Trop. Dis. Santos,GM; Kückelhaus,SA; Roselino,AM; Chaer,WK; Sampaio,RNR. The first autochthonous case of American cutaneous leishmaniasis was reported in the Federal District in 1980, and the species involved in this type of leishmaniasis was unknown. This study aimed to identify the species that causes the disease in the Federal District and to investigate its clinical and epidemiological aspects. Between 2000 and 2007, 71 autochthonous cases of leishmaniasis were reported in the Federal District. Leishmania species were identified by means of direct immunofluorescence reactions using monoclonal antibodies and restriction fragment length polymorphism. The species of 40 (56.33%) out of 71 samples were identified. Thirty-six (90%) were identified as Leishmania (Viannia) braziliensis and four (10%) were identified as Leishmania... Tipo: Info:eu-repo/semantics/other Palavras-chave: American cutaneous leishmaniasis; Leishmania Viannia braziliensis; Federal District; Autochthonous cases. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000300012 Registros recuperados: 2 Primeira ... 1 ... Última

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