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Prevalence of virulence factors in Escherichia coli strains isolated from the genital tract of healthy cows Arq. Bras. Med. Vet. Zootec.
Resende,D.; Santo,E.; Macedo,C.; Marin,J.M..
The prevalence of virulence genes expressing fimbriae, production of hemolysin, colicin and aerobactin, was determined in Escherichia coli isolates from healthy cow’s genital tract not showing clinical signs of infection. The presence of fimbriae expression genes (pap, sfa, afa) was assayed using specific primers in a polymerase chain reaction; none were detected in any of the isolates. Yet, a prevalence of 90.4%, 69.8%, and 28.5% of virulence factors for colicin, hemolysin and aerobactin respectively, was detected in the isolates. Analysis of the bacterial pathogenicity of isolates from the bovine genital tract may contribute towards the understanding of E. coli behavior.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cow; Escherichia coli; Genital tract; Virulence factors.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000300003
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Occurrence of toxigenic Escherichia coli in raw milk cheese in Brazil Arq. Bras. Med. Vet. Zootec.
Paneto,B.R.; Schocken-Iturrino,R.P.; Macedo,C.; Santo,E.; Marin,J.M..
The occurrence of toxigenic Escherichia coli in raw milk cheese was surveyed in Middle Western Brazil. Fifty samples of cheese from different supermarkets were analyzed for E.coli. The isolates were serotyped and screened for the presence of verotoxigenic E. coli (VTEC) and enterotoxigenic E. coli (ETEC) by Polymerase Chain Reaction (PCR). The susceptibility to thirteen antimicrobial agents was evaluated by the disk diffusion method. E.coli were recovered from 48 (96.0%) of the samples. The serogroups identified were O125 (6.0%), O111 (4.0%), O55 (2.0%) and O119 (2.0%). Three (6.0%) and 1(2.0%) of the E.coli isolates were VTEC and ETEC, respectively. Most frequent resistance was observed to the following antimicrobials: cephalothin (60.0%), nalidixic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Cheese; VTEC; ETEC; Antimicrobial resistance.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200035
Registros recuperados: 2
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