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Production of specific polyclonal antibodies anti-human factor IX BABT
Chaves,Daniel Gonçalves; Rodrigues,Cibele Velloso; Ferreira,Wanderley Almeida; Siqueira,Pollyana Fantini Miranda; Duarte,Clara Guerra; Carneiro-Proietti,Anna Bárbara de Freitas; Santoro,Marcelo Matos.
In this work, polyclonal antibodies anti-human Factor IX were produced in New Zealand rabbits by immunization with commercial pure human FIX (hFIX) (Octanyne®, Octapharma, USA). The serum containing immunoglobulins anti-hFIX was useful to detect hFIX antigen in human plasma fractions submitted to anionic exchange chromatographic process and with a large yield. Immunoassays (ELISA) using bovine serum albumin, trypsin and peptides generated by cleavage assays with trypsin as digestion enzyme was performed and revealed adequate specificity of the polyclonal antibodies produced.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Factor IX; Hemophilia B; HPLC; Purification; Antibodies; ELISA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500010
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Determination of optimal glucose concentration for microcalorimetric metabolic evaluation of equine spermatozoa BABT
Vasconcelos,André Belico de; Souza,Patrícia Castanheira de; Varago,Fabiana Cristina; Lagares,Monique de Albuquerque; Santoro,Marcelo Matos.
The heat conduction microcalorimeter can be used to evaluate the metabolic rates of the sperm cell. Two ejaculates of four stallions were cooled to +5ºC and checked for sperm motility (bright field microscopy), viability (eosin 3%), functional membrane integrity (hyposmotic swelling test), and heat production (microcalorimetry). Glucose and sperm cell concentrations were determined in order to measure the heat outputs resulting from sperm metabolism. Sperm viability, membrane integrity and sperm motility did not differ among the different glucose concentrations tested. Nevertheless, the highest heat output detected by the microcalorimeter was obtained with 6 mM glucose and 10(8) spermatozoa/mL. Since conduction microcalorimetry offered additional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microcalorimetry; Glucose; Spermatozoa; Sperm metabolism; Cell viability; Equine.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010
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Improved purification process of β- and α-trypsin isoforms by ion-exchange chromatography BABT
Santos,Alexandre Martins Costa; Oliveira,Jamil Silvano de; Bittar,Eustáquio Resende; Silva,Anderson Lourenço da; Guia,Marcos Luiz dos Mares; Bemquerer,Marcelo Porto; Santoro,Marcelo Matos.
The purpose of this work was to improve the separation and yield of pure β- and α-trypsin isoforms by ion-exchange chromatography and to characterize some physical-chemical properties of these isoforms. Purification of trypsin isoforms was performed by ion-exchange chromatography in 0.1 mol/L tris-HC buffer, pH 7.10 at 4ºC. The sample loading, salt concentration, flow rate and pH of mobile phase were varied to determine their effects on the resolution of the separation. The resolution was optimized mainly between β- and α-trypsin. Pure isoforms were obtained by chromatographying 100 mg of commercial trypsin during seven days, yielding 51 mg of high purity β-trypsin and 13 mg of α-trypsin partially pure, with small amounts of contaminating of ψ-trypsin....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypsin isoforms; Resolution; Ion-exchange chromatography; Purification; Specific activity; Mass spectrometry.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000400009
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Efeito de parâmetros hidrolíticos na obtenção de hidrolisados proteicos de farinha de trigo com baixo teor de fenilalanina Ciênc. Tecnol. Aliment.
Carreira,Raquel Linhares; Ramos,Camila Salles; Vasconcelos,André Belico de; Santoro,Marcelo Matos; Silvestre,Marialice Pinto Coelho.
Tendo como objetivo a obtenção de hidrolisados proteicos de farinha de trigo com baixo teor de fenilalanina (Phe), foram preparados, inicialmente, extratos proteicos da farinha de trigo, empregando-se método enzimático pela ação de protease de Bacillus licheniformis. Em seguida, esses extratos foram hidrolisados sob a ação do extrato enzimático bruto (EEB), obtido de casca de abacaxi, e de pancreatina comercial; e alguns parâmetros hidrolíticos foram avaliados, tais como temperatura (30; 35; 40; 50; e 70 °C), tempo (1 hora e 30 minutos; 2 horas e 30 minutos; 3 horas e 30 minutos), e pH de reação (6,0; 7,0; 8,0 e 9,0). Para a remoção de Phe, empregou-se o carvão ativado (CA) e a eficiência deste processo foi avaliada determinando-se o teor de Phe por...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Farinha de trigo; Extração proteica; Hidrólise; Enzimas; Fenilalanina.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000100023
Registros recuperados: 4
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