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| Cavalheiro,Norma de Paula; Santos,Ana Carolina de Oliveira; Melo,Carlos Eduardo; Morimitsu,Suzana Rie; Barone,Antonio Alci. |
| Though HCV infection is a serious public health problem, some aspects of its biology are still not well understood, such as its transmission through seminal fluid and sexual transmission. We looked for HCV in the semen of infected patients. Thirteen patients were included. Semen fractions (seminal plasma, leukocytes and spermatozoa) were separated with 45% and 90% Percoll gradients. The HCV-RNA in blood and semen fractions was extracted using the same protocol (AMPLICOR Roche) and was detected using the qualitative Roche Amplicor test and by agarose gel electrophoresis, with ethidium bromide staining. The mean age of the patients was 40.7 years. Risk factors for the acquisition of HCV included injectable and inhaled drug use in six (42.8%), blood... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HCV; PCR; Sexual transmission; Semen. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000500003 |
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