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Santos,Anderson F.; Pires,Fabiano; Jesus,Hugo E.; Santos,André L.S.; Peixoto,Raquel; Rosado,Alexandre S.; D'Avila-Levy,Claudia M.; Branquinha,Marta H.. |
Two psychrophilic bacterial samples were isolated from King George Island soil, in Antarctica. The phylogenetic analysis based on the 16S rRNA (rrs) gene led to the correlation with the closest related isolates as Sporosarcina aquimarina (99%) and Algoriphagus antarcticus(99%), with query coverage of 99% and 98%, respectively.The spent culture media from both isolates displayed proteolytic activities detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis containing gelatin as protein substrate. Under the employed conditions, S. aquimarina showed a 55 kDa protease with the best activity detected at pH 7.0 and at 27°C. A. antarcticusalso showed a single extracellular protease, however its molecular mass was around 90kDa and its best activity... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Algoriphagus antarcticus; Antarctica; Sporosarcina aquimarina; Protease. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100109 |
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Santos,Anderson F.; Valle,Roberta S.; Pacheco,Clarissa A.; Alvarez,Vanessa M.; Seldin,Lucy; Santos,André L.S.. |
Halophilic microorganisms are source of potential hydrolytic enzymes to be used in industrial and/or biotechnological processes. In the present study, we have investigated the ability of the moderately halophilic bacterium Halobacillus blutaparonensis (strain M9), a novel species described by our group, to release proteolytic enzymes. This bacterial strain abundantly proliferated in Luria-Bertani broth supplemented with 2.5% NaCl as well as secreted proteases to the extracellular environment. The production of proteases occurred in bacterial cells grown under different concentration of salt, ranging from 0.5% to 10% NaCl, in a similar way. The proteases secreted by H. blutaparonensis presented the following properties: (i) molecular masses ranging from 30... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Halobacillus blutaparonensis; Halophilic bacterium; Serine protease. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400039 |
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