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	Provedor de dados BJM (2) Autor Oliveira,Valéria Maia de (2) Santos Neto,Eugênio Vaz dos (2) Dellagnezze,Bruna Martins (1) Melo,Itamar Soares de (1) Sette,Lara Durães (1) Simioni,Karen Christina Marques (1) Vasconcellos,Suzan Pantaroto de (1) Mais... Palavra-chave Bacterial diversity (2) 16S rDNA (1) 16S rRNA (1) Community DNA (1) Cultivation-independent analysis (1) Microbial enrichment (1) Petroleum (1) Petroleum reservoir (1) Physical support (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2016 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Evaluation of bacterial diversity recovered from petroleum samples using different physical matrices BJM Dellagnezze,Bruna Martins; Vasconcellos,Suzan Pantaroto de; Melo,Itamar Soares de; Santos Neto,Eugênio Vaz dos; Oliveira,Valéria Maia de. ABSTRACT Unraveling the microbial diversity and its complexity in petroleum reservoir environments has been a challenge throughout the years. Despite the techniques developed in order to improve methodologies involving DNA extraction from crude oil, microbial enrichments using different culture conditions can be applied as a way to increase the recovery of DNA from environments with low cellular density for further microbiological analyses. This work aimed at the evaluation of different matrices (arenite, shale and polyurethane foam) as support materials for microbial growth and biofilm formation in enrichments using a biodegraded petroleum sample as inoculum in sulfate reducing condition. Subsequent microbial diversity characterization was carried out... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Microbial enrichment; Petroleum reservoir; Physical support; 16S rRNA. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000300712 Bacterial diversity characterization in petroleum samples from Brazilian reservoirs BJM Oliveira,Valéria Maia de; Sette,Lara Durães; Simioni,Karen Christina Marques; Santos Neto,Eugênio Vaz dos. This study aimed at evaluating potential differences among the bacterial communities from formation water and oil samples originated from biodegraded and non-biodegraded Brazilian petroleum reservoirs by using a PCR-DGGE based approach. Environmental DNA was isolated and used in PCR reactions with bacterial primers, followed by separation of 16S rDNA fragments in the DGGE. PCR products were also cloned and sequenced, aiming at the taxonomic affiliation of the community members. The fingerprints obtained allowed the direct comparison among the bacterial communities from oil samples presenting distinct degrees of biodegradation, as well as between the communities of formation water and oil sample from the non-biodegraded reservoir. Very similar DGGE band... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diversity; Community DNA; Cultivation-independent analysis; Petroleum; 16S rDNA. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300007 Registros recuperados: 2 Primeira ... 1 ... Última

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